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Products:Cardiovascular >> Blood >> Coagulation >> Intrinsic
Overview
Product name
Anti-Factor X antibody
See all Factor X products (12) ...
Description
Mouse monoclonal to Factor X
Specificity
This antibody recognizes the heavy chain of factor X and Xa, BEGRck factor Xa.
Tested applications
WB, ELISA, RIAmore details
Cross reactivity
Reacts with
Sheep, Rabbit, Cow, Dog, Human, Pig
Immunogen
Full length native purified Cow Factor X
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% (v/v)Glycerol/water
Concentration
Concentration information loading...
Purity
Ion Exchange Chromatography
Purification notes
Salt fractionation followed by gel filtration and ion exchange chromatography.
Clonality
Monoclonal
Isotype
IgG1
Research areas
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Cardiovascular >> Blood >> Coagulation >> Intrinsic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 55 kDa.
ELISA: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
Target
Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificity
Plasma; synthesized in the liver.
Involvement in disease
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
Post-translational
modifications
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Cellular localization
Secreted.
Target information above from: UniProt accessionP00742
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
Cleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain
Alternative names
- Activated factor Xa heavy chain antibody
- Coagulation factor antibody
- Coagulation factor X antibody
see all
Database links
- Entrez Gene: 280787 Cow
- Entrez Gene: 476993 Dog
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00743 Cow
- SwissProt: P00742 Human
- SwissProt: O19045 Rabbit
- Unigene: 361463 Human
References for Anti-Factor X antibody (ab61362)
ab61362 has not yet been referenced specifically in any publications.
Publishing research using ab61362? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"