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Product name
Anti-Factor X antibody
See all Factor X products (12) ...
Description
Rabbit polyclonal to Factor X
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Monkey
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 33 and 282 of Human Factor X (NP_000495).
Positive control
HeLa and HepG2 cell lysates
Properties
Form
Liquid
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Cardiovascular >> Blood >> Coagulation >> Intrinsic
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Western blot - Factor X antibody (ab97632)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab97632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
Target
Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificity
Plasma; synthesized in the liver.
Involvement in disease
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
Post-translational
modifications
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Cellular localization
Secreted.
Target information above from: UniProt accessionP00742
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
Cleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain
Alternative names
- Activated factor Xa heavy chain antibody
- Coagulation factor X antibody
- F10 antibody
see all
Database links
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
Anti-Factor X antibody images:
Western blot - Factor X antibody (ab97632)
Anti-Factor X antibody (ab97632) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size : 55 kDa
10% SDS PAGE
References for Anti-Factor X antibody (ab97632)
ab97632 has not yet been referenced specifically in any publications.
Publishing research using ab97632? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"