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Products:Cardiovascular >> Blood >> Coagulation >> Intrinsic
Overview
Product name
Factor X protein
See all Factor X products (6) ...
Protein description
Full length native Factor X protein (purified) (Human).
Properties
Purity
Immunogen affinity purified
Purification notes
Human Factor X is isolated from fresh frozen human plasma by a combination of conventional techniques and immunoaffinity chromatography. Purity was determined by SDS-PAGE analysis.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 50% H2O
Concentration
Concentration information loading...
Research areas
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Cardiovascular >> Blood >> Coagulation >> Intrinsic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE
Functional Studies
Application notes
Activity was measured in a factor X clotting assay.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificity
Plasma; synthesized in the liver.
Involvement in disease
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
Post-translational
modifications
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Cellular localization
Secreted.
Target information above from: UniProt accessionP00742
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for Factor X protein (ab62549)
ab62549 has not yet been referenced specifically in any publications.
Publishing research using ab62549? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"