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Overview

  • Product nameFactor XI Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.56 ng/ml - 50 ng/ml
  • Recovery

    97.5 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108834 is designed for detection of Fuman Factor XI in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Factor XI in less than 4 hours. A polyclonal antibody specific for Factor XI has been pre-coated onto a 96-well microplate with removable strips. Factor XI in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for FXI, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionFactor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
  • Tissue specificityIsoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
  • Involvement in diseaseFactor XI deficiency (FA11D) [MIM:612416]: A hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications    Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P03951 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Coagulation factor XICoagulation factor XIa light chainF11
        FA11_HUMANFXIPlasma thromboplastin antecedentPTA
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108834 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Factor XI Human ELISA Kit images

      • Sandwich ELISA - Factor XI Human ELISA Kit (ab108834)
        Sandwich ELISA - Factor XI Human ELISA Kit (ab108834)
        Example standard curves generated using ab108834. A standard curve should be generated each time the assay is performed.

      Protocols

      References for Factor XI Human ELISA Kit (ab108834)

      ab108834 has not yet been referenced specifically in any publications.

      Publishing research using ab108834 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108834 Submit a Question for ab108834

      Displaying 1 - 3 of 3 results for Abreviews and Q&A

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      Votes: Highest First

      Dear Josephine



      Thanks for your kindly reply, this customer also wants to know the retention period of the “Factor XI standard”. Could you please help this customer to solve the problem?



      Thanks for ...

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      Thank you for contacting us.

      1. At the power form, it should be good for around 1 year.
      2. After dissolve in buffer, the standard need to be aliquot and store at -80 degree if possible. If store at -80, the standard can be good for at least...

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      Product code: 108834

      Inquiry: Dear technical support team: This customer raised an enquire about ab108834 (Factor XI Human ELISA Kit), he wants to know the retention period of the “Factor XI Microplate (12 strips of 8 wells)”, could...

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      Thank you for contacting us.

      The plate can last for at least one year from the date received the kit as long as the pouch un-open. If the pouch has been opened, please keep it in the desicator if you have one. Keep dry is very important. If you ...

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      Merci de votre réponse. Toutefois elle me donne une information partielle. Ma question était " combien de dosage de facteur XI différés, dans le temps, peut on faire avec un seul Kit".? Aujourd'hui je sais que le kit n'est pas utilisable plus que deux moi...

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      Comme indiqué dans le protocol du kit ELISA facteur XI ab108834 (http://www.abcam.com/ps/products/108/ab108834/documents/ab108834%20Factor%20XI%20Human%20ELISA_v3%20%28Website%29.pdf), il est conseillé d'effectuer 2 ou 3 séries de 7 points de standards (e...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"