Factor XI Human ELISA Kit (ab108834)
- Product nameFactor XI Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 1.5 ng/ml
- Range1.56 ng/ml - 50 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
ab108834 is designed for detection of Fuman Factor XI in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Factor XI in less than 4 hours. A polyclonal antibody specific for Factor XI has been pre-coated onto a 96-well microplate with removable strips. Factor XI in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for FXI, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Factor XI antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Factor XI Microplate (12 strips of 8 wells) 1 unit Factor XI standard 2 vials Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- Research Areas
Contains 4 apple domains.
Contains 1 peptidase S1 domain.
modificationsActivated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.
- Coagulation factor XICoagulation factor XIa light chainF11
- FA11_HUMANFXIPlasma thromboplastin antecedentPTA
Our Abpromise guarantee covers the use of ab108834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Factor XI Human ELISA Kit images
Example standard curves generated using ab108834. A standard curve should be generated each time the assay is performed.
References for Factor XI Human ELISA Kit (ab108834)
ab108834 has not yet been referenced specifically in any publications.