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Overview

  • Product nameFactor XII Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 6 ng/ml
  • Range
    6.25 ng/ml - 100 ng/ml
  • Recovery

    99 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human coagulation factor XII (FXII), Hageman factor, is a plasma serine protease existing in the zymogen form. Upon contacting with negatively charged artificial or biologic surfaces, FXII is autoactivated into FXIIa that initiates intrinsic blood coagulation, fibrinolysis, and activation of the inflammatory kallikrein-kinin and complement systems. FXII has 615 amino acids, weighs 80 kDa and circulates in normal plasma at a concentration of 30 µg/ml. It is a multidomain protein with structure similarity to EGF, single chain urokinase, and tissue plasminogen activator. In the intravascular compartment, FXII binds to endothelial cell urokinase plasminogen activator receptor, cytokeratin 1, and the complement receptor. FXII deficiency or blockade protects from cerebral ischemia without overtly affecting hemostasis. FXII inhibition could be a novel target for safer anticoagulation and stroke prevention without the side effect of increased bleeding.

    ab108835 is designed for detection of Human factor XII in plasma, serum, milk, urine, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures factor XII in less than 4 hours. A murine antibody specific for factor XII has been pre-coated onto a 96-well microplate with removable strips. Factor XII in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for factor XII, which is recognized by a streptavidinperoxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108835 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Factor XII Human ELISA Kit images

      • Sandwich ELISA - Factor XII Human ELISA Kit (ab108835)
        Sandwich ELISA - Factor XII Human ELISA Kit (ab108835)
        Example of standard curve obtained using ab108835

      Protocols

      References for Factor XII Human ELISA Kit (ab108835)

      ab108835 has not yet been referenced specifically in any publications.

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      Can these reactions be done at room temperature instead of 37C? Can I just analyze the end points or will this be less accurate?

      Thank you for your call last week and for your patience. I apologize for the extended delay in getting this information for you.

      I've just received a reply from the lab, and these kits can be analyzed at room temperature. The OD might be slightl...

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