Factor XII Human ELISA Kit (ab108835)
- Product nameFactor XII Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 5% Inter-assay Sample n Mean SD CV% Overall 7.1%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Milk, Urine, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity0.02 ng/ml
- Range6.25 ng/ml - 100 ng/ml
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Factor XII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XII concentrations in plasma, serum, milk, urine and cell culture supernatants.
A Factor XII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XII captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor XII Antibody 1 x 140µl Chromogen Substrate 1 x 8ml Factor XII Microplate (12 x 8 well strips) 1 unit Factor XII Standard 1 unit Sealing Tapes 3 units Stop Solution 1 x 12ml
- Research Areas
- SwissProt: P00748 Human
Our Abpromise guarantee covers the use of ab108835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Factor XII Human ELISA Kit (ab108835)
ab108835 has not yet been referenced specifically in any publications.