|
|
|
|
|
Product Name
|
Factor XII heavy chain antibody [801]
|
| |
See all Factor XII heavy chain antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [801] to Factor XII heavy chain
|
|
Immunogen
|
Full length native protein (purified) (Human).
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
Reacts with the heavy chain of human factor XII (mw~50,000) with KD of 9.8nM and partially blocks Factor XII activity.
|
|
Epitope
|
The specific epitope is located within a 20 amino acid sequence of 2.5kD in the surface binding area of Factor XII.
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Dilution optimised using Chromogenic detection. Coagulation assays and immunopurification of Factor XII. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Cardiovascular >> Blood >> Coagulation >> Intrinsic
|
|
Relevance
|
Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in Factor XII gene do not cause any clinical symptoms and the sole effect is that whole blood clotting time is prolonged.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
801
|
|
Isotype
|
IgG1
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: None
Constituents: PBS, pH 7.4
|
|
Form
|
Liquid
|
|
Concentration
|
0.660 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Factor XII heavy chain antibody [801] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to Factor XII heavy chain
|
| |
Factor XII heavy chain antibody [801] - more information
|
Customer reviews (feedback) regarding Factor XII heavy chain antibody [801] |
Customer FAQs regarding Factor XII heavy chain antibody [801] |
Protocols for Factor XII heavy chain antibody [801] |
Price and availability of products related to Factor XII heavy chain antibody [801] |
| |
Factor XII heavy chain antibody [801] - related products:
|
|
|
| |
Factor XII heavy chain antibody [801] - other tools:
|
Contact Abcam with a Technical Enquiry about Factor XII heavy chain antibody [801] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
