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Products:Cardiovascular >> Blood >> Coagulation >> Intrinsic
Overview
Product name
Anti-Factor XII heavy chain antibody [801]
See all Factor XII heavy chain products (3) ...
Description
Mouse monoclonal [801] to Factor XII heavy chain
Specificity
Reacts with the heavy chain of human factor XII (mw~50,000) with KD of 9.8nM and partially blocks Factor XII activity.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native protein (purified) (Human).
Epitope
The specific epitope is located within a 20 amino acid sequence of 2.5kD in the surface binding area of Factor XII.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
801
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Cardiovascular >> Blood >> Coagulation >> Intrinsic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Dilution optimised using Chromogenic detection. Coagulation assays and immunopurification of Factor XII. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in Factor XII gene do not cause any clinical symptoms and the sole effect is that whole blood clotting time is prolonged.
Cellular localization
Secreted
Alternative names
- Coagulation factor XII antibody
- Coagulation factor XIIa heavy chain antibody
- F12 antibody
see all
Database links
- Entrez Gene: 2161 Human
- Omim: 610619 Human
- SwissProt: P00748 Human
References for Anti-Factor XII heavy chain antibody [801] (ab20797)
ab20797 has not yet been referenced specifically in any publications.
Publishing research using ab20797? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"