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Western blot - Factor XII heavy chain antibody [B7C9] (ab1007)
![Western blot - Factor XII heavy chain antibody [B7C9] (ab1007)](/ps/datasheet/images/ab1007_1.jpg)
(enlarge)
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Product Name
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Factor XII heavy chain antibody [B7C9]
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See all Factor XII heavy chain antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [B7C9] to Factor XII heavy chain
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Immunogen
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Purified human Factor XII.
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Reacts with
(species key)
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Hu, Rat
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Specificity
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This antibody reacts with the heavy chain of human Factor XII (mw~50,000) with a KD of 9.8nM.
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Epitope
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The specific epitope for this antibody is located within a 20 amino acid sequence in the surface binding area of Factor XII.
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Tested applications
(see key)
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AP, ELISA, IP, Inhib, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dilute in PBS or medium which is identical to that used in the assay system.
This antibody partially blocks Factor XII activity.
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Cellular localization
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Secreted
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Research areas
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Cardiovascular >> Blood >> Coagulation >> Intrinsic
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Relevance
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Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in Factor XII gene do not cause any clinical symptoms and the sole effect is that whole blood clotting time is prolonged.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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B7C9
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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PBS, pH7.2
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Form
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Liquid
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Concentration
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1.300 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Factor XII heavy chain antibody [B7C9] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Factor XII heavy chain antibody [B7C9] images, references, products related to ab1007 and other tools.
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Factor XII heavy chain antibody [B7C9] images:
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 Western blot - Factor XII heavy chain antibody [B7C9] (ab1007)
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 48,70 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
ab1007 at 1/2000 staining Factror XII heavy chain by Western Blot. Lanes 1-6: Human vitreous tissue lysate (2 ug/lane). Lane 7: Factor XII Lane 8: Factor XIIa. A HRP conjugated goat anti-mouse antibody was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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References for Factor XII heavy chain antibody [B7C9] (ab1007)
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This product has been used in: (two most recent references)
Gao BB et al. Extracellular carbonic anhydrase mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation. Nat Med 13:181-8 (2007). PubMed: 17259996
Pixley RA et al. A monoclonal antibody recognizing an icosapeptide sequence in the heavy chain of human factor XII inhibits surface-catalyzed activation. J Biol Chem 262:10140-5 (1987). PubMed: 2440859
If you publish research using ab1007 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Factor XII heavy chain
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Factor XII heavy chain antibody [B7C9] - more information
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Customer reviews (feedback) regarding Factor XII heavy chain antibody [B7C9] |
Customer FAQs regarding Factor XII heavy chain antibody [B7C9] |
Protocols for Factor XII heavy chain antibody [B7C9] |
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Factor XII heavy chain antibody [B7C9] - related products:
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Factor XII heavy chain antibody [B7C9] - other tools:
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Contact Abcam with a Technical Enquiry about Factor XII heavy chain antibody [B7C9] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Factor XII heavy chain antibody [B7C9] (ab1007)](/index.html?datasheet=1007#ab1007_1.jpg)
