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Products:Cardiovascular >> Blood >> Coagulation >> Intrinsic
Overview
Product name
Anti-Factor XII heavy chain antibody
See all Factor XII heavy chain products (3) ...
Description
Rabbit polyclonal to Factor XII heavy chain
Specificity
ab72633 detects activated Factor XII heavy chain, resulting from cleavage adjacent to Arg372.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from human Factor XII heavy chain.
Positive control
Extracts from 293 cells treated with etoposide (25µM, 1hour).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Coagulation >> Intrinsic
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Western blot - Factor XII heavy chain ( R372) antibody (ab72633)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72633 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight of fragment: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in Factor XII gene do not cause any clinical symptoms and the sole effect is that whole blood clotting time is prolonged.
Cellular localization
Secreted
Alternative names
- Coagulation factor XII antibody
- Coagulation factor XII heavy chain antibody
- Coagulation factor XIIa heavy chain antibody
see all
Database links
- Entrez Gene: 2161 Human
- Omim: 610619 Human
- SwissProt: P00748 Human
Anti-Factor XII heavy chain antibody images:
Western blot - Factor XII heavy chain ( R372) antibody (ab72633)
All lanes : Anti-Factor XII heavy chain antibody (ab72633) at 1/500 dilution
Lane 1 : extracts from 293 cells treated
with etoposide (25µM, 1hour)
Lane 2 : extracts from 293 cells treated
with etoposide (25µM, 1hour), plus immunising peptide at 5µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 42 kDa
Observed band size : 42 kDa
References for Anti-Factor XII heavy chain antibody (ab72633)
ab72633 has not yet been referenced specifically in any publications.
Publishing research using ab72633? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"