Factor XIII Human ELISA Kit (ab108836)
- Product nameFactor XIII Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 2.5 ng/ml
- Range5 ng/ml - 50 ng/ml
- Assay time4h 0m
- Species reactivityReacts with: Human
- Product overview
Factor XIII is a proenzyme for a plasma transglutaminase, previously known as fibrin stabilizing factor. Intracellular FXIII exists as a dimer of two FXIIIA molecules, whereas the circulating plasma FXIII is composed of two FXIIIA and two FXIIIB subunits. This tetramer is activated in the presence of thrombin and Ca2+ by separation of the two subunits and cleavage of the 37 amino acid activation peptide from the N-terminal of the FXIIIA molecule. Inherited Factor XIII deficiency can result from mutations in either the A- or B- subunit genes. Factor XIIIA subunit deficiency is an autosomal recessive disorder that is characterized by a life-long bleeding tendency and complications in wound healing.
ab108836 Factor XIII Human ELISA kit is designed for detection of Human Factor XIII in plasma, serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures FXIII in less than 4 hours. A murine antibody specific for FXIII has been pre-coated onto a 96-well microplate with removable strips. FXIII in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for FXIII, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Factor XIII Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Factor XIII Microplate( 12 strips of 8 wells) 1 unit Factor XIII Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
Our Abpromise guarantee covers the use of ab108836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Factor XIII Human ELISA Kit (ab108836)
ab108836 has not yet been referenced specifically in any publications.