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Overview

  • Product nameFactor XIII Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 2.5 ng/ml
  • Range
    5 ng/ml - 50 ng/ml
  • Recovery

    99 %

  • Assay time
    4h 0m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Factor XIII is a proenzyme for a plasma transglutaminase, previously known as fibrin stabilizing factor. Intracellular FXIII exists as a dimer of two FXIIIA molecules, whereas the circulating plasma FXIII is composed of two FXIIIA and two FXIIIB subunits. This tetramer is activated in the presence of thrombin and Ca2+ by separation of the two subunits and cleavage of the 37 amino acid activation peptide from the N-terminal of the FXIIIA molecule. Inherited Factor XIII deficiency can result from mutations in either the A- or B- subunit genes. Factor XIIIA subunit deficiency is an autosomal recessive disorder that is characterized by a life-long bleeding tendency and complications in wound healing.

    ab108836 Factor XIII Human ELISA kit is designed for detection of Human Factor XIII in plasma, serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures FXIII in less than 4 hours. A murine antibody specific for FXIII has been pre-coated onto a 96-well microplate with removable strips. FXIII in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for FXIII, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108836 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Factor XIII Human ELISA Kit images

      • ELISA - Factor XIII Human ELISA Kit (ab108836)
        ELISA - Factor XIII Human ELISA Kit (ab108836)
        Example Standard Curve

      Protocols

      References for Factor XIII Human ELISA Kit (ab108836)

      ab108836 has not yet been referenced specifically in any publications.

      Publishing research using ab108836 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108836 Submit a Question for ab108836

      Displaying 1 - 6 of 6 results for Abreviews and Q&A

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      we would like you to specify what the ELISA antibody binds to, please read the below email.

      Thank you for contacting us.

      The kit using different antibodies, but the binding site is not mapped. Since they both polyclonal antibody, it may have multiple binding sites for protein.


      I hope this information is helpful to you. Ple...

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      Does this kit detect both the Factor XIIIA and B?

      Thank you for contacting us.


      Yes, the kit will detect both the A and B forms.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      How can I get a discount from ordering in bulk?

      Thanks for your enquiry!

      Please contact our bulk order specialist, Andrea Gray at mailto:Andrea.Gray@abcam.com, regarding discounts on bulk order purchases.

      I hope this is helpful. Please contact me again if you have any further questi...

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      The OD's for the standard curve were very low- the highest standard measured at 0.21. Background was 0.046. Samples read around 0.1 (double the background). This seems very low, do you agree? After the streptavidin-peroxidase incubation, the plates were w...

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      Thank you for your call last week and for your patience while I've been in touch with the lab regarding your questions.

      I've heard back from my contact at the lab, and she agrees that the OD values are lower than expected. An OD of 1.5-2.0 would...

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      needs an antibody/ELISA kit which would recongize the first 37 amino acids of the Factor XIII

      Thank you for your call the other day.

      I am happy to confirm that the kit ab108836 against the human Factor XIII will recognize the full length protein as well as the fragment of the 37 first amino acids. I have just received the confirmation fr...

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      Which cleavage products will ab108836 detect? There are 3, 80kDa, 56kDa and 24kDa.

      This ELISA kit will recognize factor XIIIa and factor XIII.  I hope this is helpful. Please contact me again if you have any further questions.  

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"