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ab4057 staining formalin fixed paraffin-embedded human bowel tumour tissue sections. The sample was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% srum for 1 hour at 24°C, prior to incubation with the primary antibody (diluted 1/100 in milk) for 12 hours at 4°C. A biotinylated goat anti-rabbit antibody was used as the secondary.
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