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- Secondary antibodies
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Product name
Anti-Factor Xa Heavy Chain antibody
See all Factor Xa Heavy Chain products (2) ...
Description
Rabbit polyclonal to Factor Xa Heavy Chain
Specificity
Detects endogenous levels of fragment of Factor Xa resulting from cleavage adjacent to Ile235
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from human Factor Xa heavy chain.
Positive control
Extracts from rat liver cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Cardiovascular >> Blood >> Coagulation >> Intrinsic
-
Western blot - Factor Xa Heavy Chain antibody (ab75180)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: 1/20000.
WB: 1/500 - 1/1000. Predicted molecular weight: 55 kDa (uncleaved precursor).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificity
Plasma; synthesized in the liver.
Involvement in disease
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
Post-translational
modifications
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Cellular localization
Secreted.
Target information above from: UniProt accessionP00742
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activated factor Xa heavy chain antibody
- Coagulation factor X antibody
- EC 3.4.21.6 antibody
see all
Database links
- Entrez Gene: 2159 Human
- Entrez Gene: 14058 Mouse
- Entrez Gene: 29243 Rat
- Omim: 227600 Human
- SwissProt: P00742 Human
- SwissProt: O88947 Mouse
- SwissProt: Q63207 Rat
- Unigene: 361463 Human
see all
Anti-Factor Xa Heavy Chain antibody images:
Western blot - Factor Xa Heavy Chain antibody (ab75180)
All lanes : Anti-Factor Xa Heavy Chain antibody (ab75180) at 1/500 dilution
Lane 1 : extracts from rat liver cells
Lane 2 : extracts from rat liver cells with immunizing peptide at 5 µg
Lysates/proteins at 10 µg per lane.
Predicted band size : 55 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
References for Anti-Factor Xa Heavy Chain antibody (ab75180)
ab75180 has not yet been referenced specifically in any publications.
Publishing research using ab75180? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"