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Fas Ligand (APTL) Human ELISA Kit (ab100515)

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Overview

  • Product nameFas Ligand (APTL) Human ELISA KitSee all Fas Ligand kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 2 pg/ml
  • Range
    1.37 pg/ml - 1000 pg/ml
  • Recovery

    75 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 76.99 70% - 90%
    Serum 76.36 70% - 87%
    Plasma 73.42 67% - 86%

  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand in serum, plasma, cell culture supernatants.

     

    This assay employs an antibody specific for human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSandwich ELISA more details

Properties

  • RelevanceFas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
  • Cellular localizationCell Membrane (single-pass type II membrane protein) and Secreted
    • Alternative names
        Apoptosis (APO 1) antigen ligand 1Apoptosis antigen ligandAPT1LG1
        APTLCD178CD95 LCD95 ligandCD95LCD95L proteinFas antigen ligandFASLFASLGTNF superfamily member 6TNFSF6Tumor necrosis factor (ligand) superfamily member 6Tumor necrosis factor ligand superfamily member 6
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100515 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Fas Ligand (APTL) Human ELISA Kit images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100515.

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100515.

      Protocols

      References for Fas Ligand (APTL) Human ELISA Kit (ab100515)

      ab100515 has not yet been referenced specifically in any publications.

      Publishing research using ab100515 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100515 Submit a Question for ab100515

      Displaying 1 - 6 of 6 results for Abreviews and Q&A

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      Votes: Highest First

      Suggested sample dilutions?

      We would suggest testing a range of dilutions to find a dilution within the standard curve. A good range is neat, 1:5, 1:10, 1:20. In the protocol the suggested dilution for normal serum/plasma: 2-10 fold, again this needs to be optimized.

      Please, I want to ask if you can send to me the optical density (OD) for each standard after serial dilution and standard curve of these readings and its equation;
      stock solution (S1) of 1000 pg/ml= OD ?
      S2 (333.3 pg/ml)= OD?
      S3 (111.1 pg/ml...

      Read More

      Unfortunately, the OD standard is never twice the same, therefore, it is so important to run a fresh standard curve for EVERY assay. The OD needs to be measured, it cannot be not calculated.
      How to analyze the data is described under section 8, page 1...

      Read More


      In this kit, where on the FasL protein do the antibodies bind? I would like to measure the amount of a chimeric FasL molecule I have made versus the WT FasL protein. My chimeric protein contains AA 139-281 of human transmembrane FasL. It also has ...

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      The exact epitopes are not known but the antibody pair is binding somewhere on the extracellular domain (aa 134-281). If it helps, the capture antibody is monoclonal and the detection antibody is polyclonal. We do not have a kit against HA although we hav...

      Read More

      If I use [CD95 Human ELISA Kit (ab100513) and Fas Ligand Human ELISA Kit (ab100515)] kits, the results represent soluble Fas and FasL or both membrane bound and soluble Fas and FasL in human serum and saliva?

      I can confirm that these kits will detect both soluble and membrane bound Fas and fas ligand.

      In order to prevent membrane bound protein to interfere with your results, I suggest to spin down the saliva samples to get rid of all cells while taki...

      Read More

      My samples are serum and saliva; and i deal with soluble human Fas and Fas Ligand.

      My question, what are the differences between [Fas Ligand Human ELISA Kit (ab45892) and Fas Ligand Human ELISA Kit (ab100515)] and what are the differences betwe...

      Read More

      I have reviewed the details on the datasheets for these kits. For the Fas ligand kits:

      ab45892 Fas Ligand Human ELISA Kit
      ab100515 Fas Ligand Human ELISA Kit

      Sensitivities are different:ab100515 < 2 pg/ml, ab45892 < 12 pg/ml Read More

      My samples are serum and saliva; and i deal with soluble human Fas and Fas Ligand.

      My question, what are the differences between [Fas Ligand Human ELISA Kit (ab45892) and Fas Ligand Human ELISA Kit (ab100515)] and what are the differences betwe...

      Read More

      These ELISA kits are from two different sources but they are similar.

      I recommend to determine which is the best for your needs from the datasheet and protocol information. Please take not of the different ranges these kits are suitable for. I s...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"