Fas Ligand Human ELISA Kit (ab45892)

Overview

• Product nameFas Ligand Human ELISA KitSee all Fas Ligand kits ...
• Detection methodColorimetric
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Sample A	40	588.4pg/ml	22.6	3.8%
  Sample B	35	302.2pg/ml	14.6	4.5%
• Tests
  1 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  < 12 pg/ml
• Range
  62.5 pg/ml - 2000 pg/ml
• Assay time
  3h 45m
• Assay durationMultiple steps standard assay
• Species reactivity
  Reacts with: Human
  
• Product overview

  INTENDED USE

  The Human soluble Fas Ligand ELISA is to be used for the in-vitro quantitative determination of soluble human Fas Ligand in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human Fas Ligand. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  PRINCIPLE OF THE METHOD

  The Fas Ligand Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for Fas Ligand has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Fas-L concentrations and unknowns are pipetted into these wells.

  During the first incubation, the Fas Ligand antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for Fas Ligand is incubated. Then the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which acts on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Fas Ligand present in the samples.

   

  Recognizes both natural and recombinant human soluble Fas Ligand
  
  Range: 62.5 pg/ml - 2000 pg/ml
  
  Sensitivity < 44 pg/ml
  

• Notes

  Safety Information:

  • For research use only.
  • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsELISA more details
• PlatformMicroplate

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	Identifier	1 x 96 tests
  10X Standard Diluent Buffer	Black	1 x 25ml
  200X Washing Buffer	White	1 x 10ml
  Biotinylated Antibody Diluent	Red	1 x 7.5ml
  Biotinylated anti-Fas Ligand	Red	1 x 0.4ml
  Chromogen TMB		1 x 11ml
  Fas Ligand Control	Silver	2 vials
  Fas Ligand precoated microtiter plates		8 units
  Fas Ligand Standard: 2000 pg/ml	Yellow	2 vials
  HRP Diluent	Red	1 x 23ml
  Stop Reagent	Black	1 x 11ml
  Streptavidin HRP		2 x 5µl

• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Cell survival & death

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Apoptosis marker and proteins ELISA kits

  • Immunology
  • Innate Immunity
  • Cytokines
  • TNF Superfamily

  • Cell Biology
  • Apoptosis
  • Receptors
  • Death Receptors

  • Cell Biology
  • Apoptosis
  • Extracellular Signals
  • Death Ligands

Applications

Fas Ligand Human ELISA Kit images

• 

  ELISA - Human Fas Ligand ELISA kit (ab45892)
  Typical Fas L standard curve ranging from 62.5 to 2000pg/mL

Protocols

• Human Fas Ligand ELISA kit