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Overview

  • Product nameFas Ligand Human ELISA KitSee all Fas Ligand kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 40 588.4pg/ml 22.6 3.8%
    Sample B 35 302.2pg/ml 14.6 4.5%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 12 pg/ml
  • Range
    62.5 pg/ml - 2000 pg/ml
  • Assay time
    3h 45m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human soluble Fas Ligand ELISA is to be used for the in-vitro quantitative determination of soluble human Fas Ligand in

    human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human Fas Ligand. This

    kit has been configured for research use only and is not to be used in diagnostic procedures.

    PRINCIPLE OF THE METHOD

    The Fas Ligand Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for Fas-L has

    been coated onto the wells of the microtiter strips provided. Samples, including standards of known Fas Ligand concentrations and unknowns are

    pipetted into these wells.

    During the first incubation, the Fas Ligand antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for Fas Ligand is incubated. Then the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all unbound enzyme, a substrate

    solution which actes on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly

    proportional to the concentration of Fas Ligand present in the samples.

     

    Recognizes both natural and recombinant human soluble Fas Ligand

    Range: 62.5 pg/ml - 2000 pg/ml

    Sensitivity < 44 pg/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceFas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
  • Cellular localizationCell Membrane (single-pass type II membrane protein) and Secreted
    • Alternative names
        Apoptosis (APO 1) antigen ligand 1Apoptosis antigen ligandAPT1LG1
        APTLCD178CD95 LCD95 ligandCD95LCD95L proteinFas antigen ligandFASLFASLGTNF superfamily member 6TNFSF6Tumor necrosis factor (ligand) superfamily member 6Tumor necrosis factor ligand superfamily member 6
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab45907 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Fas Ligand Human ELISA Kit images

      • ELISA - Human Fas Ligand ELISA kit (ab45907)
        ELISA - Human Fas Ligand ELISA kit (ab45907)
        Typical Fas L standard curve ranging from 62.5 to 2000pg/mL

      References for Fas Ligand Human ELISA Kit (ab45907)

      ab45907 has not yet been referenced specifically in any publications.

      Publishing research using ab45907 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"