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Fas Ligand Human Elipair Kit (Reagents provided for 10 x 96 Well Plates) (ab83705)

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Overview

  • Product nameFas Ligand Human Elipair Kit (Reagents provided for 10 x 96 Well Plates)See all Fas Ligand kits ...
  • Tests
    10 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeELIPAIR
  • Sensitivity
    < 44 pg/ml
  • Range
    2000 pg/ml - 62.5 pg/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Fas L Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

    This Eli-pair set is intended to measure Fas L in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure

    may need some optimization depending on the plastic, matrix, standard range.

     

    SPECIFICITY: Recognizes both natural and recombinant soluble human Fas-Ligand

    RANGE: 62.5pg/ml - 2000pg/ml

    SENSITIVITY: <44pg/ml

  • Tested applicationsEli-pair more details

Properties

  • RelevanceFas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. The full length of FasL contains 281 amino acids, consisting of the transmembrane domain, extracellular domain, and the cytoplasmic region. A metalloproteinase cleaves the membrane-bound FasL to produce soluble Fas (sFasL), which exists as a homotrimer. The sFasL shows lower cytotoxicity than membrane bound FasL.
  • Cellular localizationCell membrane: single pass type II membrane protein. Secreted protein: may be released into the extracellular fluid, probably by cleavage form the cell surface.
    • Alternative names
        Apoptosis (APO 1) antigen ligand 1APT1LG1APTL
        CD178CD178 antigen CD95 ligand CD95L proteinFAS antigen ligandFas LFasl Fas ligand (TNF superfamily member 6)FASLGGeneralized lymphoproliferative disease Gld TNFSF6Tumor necrosis factor (ligand) superfamily member 6
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab83705 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Eli-pair EliP

      Fas Ligand Human Elipair Kit (Reagents provided for 10 x 96 Well Plates) images

      • Eli-pair - Fas Ligand Human Elipair Kit (Reagents provided for 10 x 96 Well Plates) (ab83705)
        Eli-pair - Fas Ligand Human Elipair Kit (Reagents provided for 10 x 96 Well Plates) (ab83705)
        Fas Ligand standard curve ranging from 62.5 to 2000 pg/mL

      References for Fas Ligand Human Elipair Kit (Reagents provided for 10 x 96 Well Plates) (ab83705)

      ab83705 has not yet been referenced specifically in any publications.

      Publishing research using ab83705 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"