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Overview

  • Product nameFas Ligand Rat ELISA KitSee all Fas Ligand kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 90 pg/ml
  • Range
    82.3 pg/ml - 60000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92.64 81% - 98%
    Serum 123.4 118% - 135%
    Plasma 118.9 109% - 125%

  • Species reactivity
    Reacts with: Rat
  • Product overview

    ab100759 rat Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Fas Ligand in serum, plasma and cell culture supernatants. This assay employs an antibody specific for rat Fas Ligand coated on a 96-well plate. Standards and samples are pipette into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Link to be added to SOP.

  • Tested applicationsELISA more details

Properties

  • RelevanceFas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
  • Cellular localizationCell Membrane (single-pass type II membrane protein) and Secreted
    • Alternative names
        Apoptosis (APO 1) antigen ligand 1Apoptosis antigen ligandAPT1LG1
        APTLCD178CD95 LCD95 ligandCD95LCD95L proteinFas antigen ligandFASLFASLGTNF superfamily member 6TNFSF6Tumor necrosis factor (ligand) superfamily member 6Tumor necrosis factor ligand superfamily member 6
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100759 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Fas Ligand Rat ELISA Kit images

      • ELISA - Fas Ligand Rat ELISA Kit (ab100759)
        ELISA - Fas Ligand Rat ELISA Kit (ab100759)

      Protocols

      References for Fas Ligand Rat ELISA Kit (ab100759)

      ab100759 has not yet been referenced specifically in any publications.

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