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Overview

  • Product nameFas Ligand s Human Elispot KitSee all Fas Ligand kits ...
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Cell culture extracts
  • Assay typeELISPOT
  • Species reactivity
    Reacts with: Human
  • Product overview

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    Principal:

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

     

    Recognizes natural human Fas ligand

  • Tested applicationsMeasuring secreted proteins from cells more details

Properties

  • RelevanceThe protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with Fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus.
  • Cellular localizationCell membrane; single-pass type II membrane protein. Secreted protein. May be released into the extracellular fluid, probably by cleavage form the cell surface.
    • Alternative names
        Apoptosis antigen ligand APT1LG1 APTL
        CD178CD178 antigenCD95L CD95L proteinFas antigen ligand Fas LFas ligand (TNF superfamily member 6)FASL FASLGTNFSF6 Tumor necrosis factor ligand superfamily member 6
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab62921 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISPOT ELISPOT

      References for Fas Ligand s Human Elispot Kit (ab62921)

      ab62921 has not yet been referenced specifically in any publications.

      Publishing research using ab62921 ? Please let us know so that we can cite the reference in this datasheet

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