Anti-Fascin antibody [SPM133] (ab15110)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fascin antibody [SPM133] (ab15110)

ab15110 staining Fascin in Human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Immunocytochemistry/ Immunofluorescence - Fascin antibody [SPM133] (ab15110)

Fascin staining of human monocytes-derived DC after 7 days culture with IL-4/GM-CSF.

This picture was kindly supplied as part of the review submitted by Fransisco Leon.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fascin antibody [SPM133] (ab15110)

ab15110 staining human ovarian cancinoma tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citric acid (pH 6) prior to blocking with 1% BSA for 10 minutes at RT.  The primary antibody was diluted 1/200 and incubated with the sample for 2 hours.

This image is courtesy of an Abreview submitted by Mr Carl Hobbs

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fascin antibody [SPM133] (ab15110)

ab15110 staining gut tissue sections from mouse embyo (approx. day 15) by IHC-P.  Sections were formaldehyde fixed and subjected to het mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 1% BSA for 10 at RT.  The primary antibody was diluted 1/100 and incubated with the sample for 16 hours.  A biotinylated goat anti-mouse antibody was used as the secondary.

This image shows positive staining of endothelial cells in the developing lamina propria.

This image is courtesy of an Abreview submitted by Mr Carl Hobbs

Flow Cytometry-Anti-Fascin antibody [SPM133](ab15110)

Overlay histogram showing HeLa cells stained with ab15110 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab151109, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed.