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Western blot - FABP4 antibody (ab66682)
Western blot - FABP4 antibody (ab66682)
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Product Name 

FABP4 antibody

 

See all FABP4 antibodies (15)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to FABP4

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human FABP4.

(Peptide available as ab66681.)

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.

WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following Lysates:
Mouse Skeletal Muscle Tissue
Mouse Thymus Tissue
Rat Ovary Tissue
Rat Colon Tissue
Rat Thymus Tissue
HepG2 Whole Cell
HeLa Whole Cell

Cellular localization 

Cytoplasmic and nuclear

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Stem Cells >> Mesenchymal Stem Cells >> Adipogenesis
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Binding proteins

Relevance 

Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long chain fatty acids, their coenzymes and other hydrophobic ligands and small molecules in the cytoplasm. It is thought that the role of these proteins includes fatty acid uptake, intracellular lipid transport and metabolism. FABP4 encodes the fatty acid binding protein found in adipocytes. It is a lipid transport protein and binds both long chain fatty acids and retinoic acid. It delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

 

Alternative names FABP4 antibody (ab66682)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2167    (Human)
11770    (Mouse)
79451    (Rat)

GeneCard

    

GC08M082553    (Human)

Omim

    

600434    (Human)

SwissProt

    

P15090    (Human)
P04117    (Mouse)
P70623    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.800 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FABP4 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for FABP4 antibody images, references, products related to ab66682 and other tools.

 

FABP4 antibody images:

Product image  Western blot - FABP4 antibody (ab66682)

Western blot - FABP4 antibody (ab66682)

All lanes : FABP4 antibody (ab66682) at 1 µg/ml

Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lane 2 : Thymus (Mouse) Tissue Lysate - normal tissue (ab29285)
Lane 3 : Rat Ovary Tissue Lysate
Lane 4 : Rat Colon Tissue Lysate
Lane 5 : Rat Thymus Tissue Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 15 kDa
Observed band size : 15 kDa
Additional bands at : 18 kDa (possible post-translational modification).

 
 

Search PubMed (MEDLINE) for references to FABP4

 

FABP4 antibody - more information

Customer reviews (feedback) regarding FABP4 antibody

Customer FAQs regarding FABP4 antibody

Protocols for FABP4 antibody

Price and availability of products related to FABP4 antibody

 

FABP4 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for FABP4 antibody

ab66681

  

FABP4 peptide

 

Compatible Secondaries for FABP4 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other FABP4 Antibodies

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FABP4 antibody - other tools:

Contact Abcam with a Technical Enquiry about FABP4 antibody

 

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