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Western blot - FABP4 antibody (ab66682)

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Product Name
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FABP4 antibody
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See all FABP4 antibodies (15)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FABP4
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human FABP4. (Peptide available as ab66681.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Lysates: Mouse Skeletal Muscle Tissue Mouse Thymus Tissue Rat Ovary Tissue Rat Colon Tissue Rat Thymus Tissue HepG2 Whole Cell HeLa Whole Cell
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Cellular localization
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Cytoplasmic and nuclear
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism Stem Cells >> Mesenchymal Stem Cells >> Adipogenesis Signal Transduction >> Metabolism >> Lipid metabolism Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Binding proteins
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Relevance
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Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long chain fatty acids, their coenzymes and other hydrophobic ligands and small molecules in the cytoplasm. It is thought that the role of these proteins includes fatty acid uptake, intracellular lipid transport and metabolism. FABP4 encodes the fatty acid binding protein found in adipocytes. It is a lipid transport protein and binds both long chain fatty acids and retinoic acid. It delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.800 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FABP4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FABP4 antibody images, references, products related to ab66682 and other tools.
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FABP4 antibody images:
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Western blot - FABP4 antibody (ab66682)
All lanes : FABP4 antibody (ab66682) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711) Lane 2 : Thymus (Mouse) Tissue Lysate - normal tissue (ab29285) Lane 3 : Rat Ovary Tissue Lysate Lane 4 : Rat Colon Tissue Lysate Lane 5 : Rat Thymus Tissue Lysate Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa Observed band size : 15 kDa Additional bands at : 18 kDa (possible post-translational modification).
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Search PubMed (MEDLINE) for references to FABP4
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FABP4 antibody - more information
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Customer reviews (feedback) regarding FABP4 antibody |
Customer FAQs regarding FABP4 antibody |
Protocols for FABP4 antibody |
Price and availability of products related to FABP4 antibody |
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FABP4 antibody - related products:
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FABP4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about FABP4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"