Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Binding proteins
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Concentration should be 1 mg/mL, but it is only ~0.4 mg/mL. |
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ANSWER: |
Thanks for your call today and for letting us know about the problem with this antibody concentration.
As we discussed, I am sending free of charge replacement vials of ab37267. 7 vials are in stock and will arrive Monday on the order ***, and 1 vial is being transferred from our UK office and should arrive in about a week on the order ***. If you have any data regarding this issue, please forward it to me for our investigation.
I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 15 kDa
ab37267 at 1/200 dilution detecting Fatty Acid Binding Protein 5 in human peripheral blood mononuclear cells. Goat anti-rabbit HRP was used as secondary antibody.
This image is courtesy of an anonymous Abreview
ab37267 (1µg/ml) staining Fatty Acid Binding Protein 5 in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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