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Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Binding proteins
Overview
Product name
Anti-Fatty Acid Binding Protein 5 antibody
See all Fatty Acid Binding Protein 5 products (2) ...
Description
Rabbit polyclonal to Fatty Acid Binding Protein 5
Specificity
ab37267 recognises Fatty Acid Binding Protein 5 (FABP5).
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein protein produced in E. coli (Human). It is the 15.2 kDa protein containing 135 amino acid residues. The amino acid sequence of the recombinant human FABP5 is 100% homologous to the amino acid sequence of the human FABP5.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Immunoaffinity chromatography on a column with immobilized recombinant human FABP5.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Binding proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/400 - 1/500.
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 15 kDa.
Target
Function
High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
Tissue specificity
Keratinocytes; highly expressed in psoriatic skin.
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ01469
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- epidermal antibody
- CFABP antibody
- Cutaneous fatty acid binding protein antibody
see all
Database links
- Entrez Gene: 2171 Human
- Omim: 605168 Human
- SwissProt: Q01469 Human
- Unigene: 408061 Human
Anti-Fatty Acid Binding Protein 5 antibody images:
Western blot - Fatty Acid Binding Protein 5 antibody (ab37267)
Predicted band size : 15 kDa
ab37267 at 1/200 dilution detecting Fatty Acid Binding Protein 5 in human peripheral blood mononuclear cells. Goat anti-rabbit HRP was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Fatty Acid Binding Protein 5 antibody(ab37267)
ab37267 (1µg/ml) staining Fatty Acid Binding Protein 5 in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-Fatty Acid Binding Protein 5 antibody (ab37267)
This product has been referenced in:
- Buers Iet al. TIP47, a lipid cargo protein involved in macrophage triglyceride metabolism. Arterioscler Thromb Vasc Biol 29:767-73 (2009).Read more (PubMed: 19286631) »
See 1 publication for this product
Publishing research using ab37267? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"