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Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183) contains antibodies against key enzymes of the mitochondrial fatty acid oxidation pathway for flow cytometry. The assay combines the power of single cell analysis obtained from flow cytometry with the specificity of antibody-based immunostaining to quantify protein levels in cultured cells. Cells are harvested and fixed/permeabilized in suspension, targets of interest are detected indirectly with highly specific well-characterized monoclonal antibodies that are then labeled with fluorescent antibodies.
Spin down the contents of the antibody vials upon receipt of the kit. Store all components upright at 4°C. This kit is stable for at least 6 months from receipt.
|100X ACADM Primary Antibody||1 x 0.12ml|
|100X ACADVL Primary Antibody||1 x 0.12ml|
|100X HADHA Primary Antibody||1 x 0.12ml|
|100X Normal Mouse IgG||1 x 0.12ml|
|100X Triton X-100||1 x 1.25ml|
|10X Phosphate Buffered Saline||1 x 100ml|
|400X Tween-20||1 x 4ml|
|Blocking Buffer||1 x 25ml|
Our Abpromise guarantee covers the use of ab118183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab118183 has not yet been referenced specifically in any publications.