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Read our guarantee »Overview
Product name
Fatty Acid Oxidation Human Flow Cytometry Kit
Tests
1 x 96 well plate
Sample type
Adherent cells, Suspension cells
Product overview
ab118183 contains antibodies against key enzymes of the mitochondrial fatty acid oxidation pathway for flow cytometry. The assay combines the power of single cell analysis obtained from flow cytometry with the specificity of antibody-based immunostaining to quantify protein levels in cultured cells. Cells are harvested and fixed/permeabilized in suspension, targets of interest are detected indirectly with highly specific well-characterized monoclonal antibodies that are then labeled with fluorescent antibodies.
Notes
Spin down the contents of the antibody vials upon receipt of the kit. Store all components upright at 4°C. This kit is stable for at least 6 months from receipt.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 100X ACADM Primary Antibody | 1 x 0.12ml | |
| 100X ACADVL Primary Antibody | 1 x 0.12ml | |
| 100X HADHA Primary Antibody | 1 x 0.12ml | |
| 100X Normal Mouse IgG | 1 x 0.12ml | |
| 100x Triton X-100 | P/N 8209707 | 1 x 1.25ml |
| 10x Phosphate buffered Saline | P/N 8209706 | 1 x 100ml |
| 400x Tween-20 | P/N 8209708 | 1 x 4ml |
| Blocking Buffer | 1 x 25ml |
Research areas
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Hydrolysis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Alternative names
- MS978
Applications
Show applications key
Our Abpromise guarantee covers the use of ab118183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Functional Studies
FuncSFuncS
Fatty Acid Oxidation Human Flow Cytometry Kit images:
Immunocytochemistry - Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)
Antibody specificity demonstrated by immunocytochemistry. Cells were processes with the Flow cytometry protocol as explained above. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab.
Flow Cytometry - Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)
Antibody specificity demonstrated by flow cytometry. Control (blue) and deficient cell lines (red) were targeted with the FAO antibody panel against ACADVL, ACADM and HADHA. Background fluorescence was determined with the supplied negative control (black). After background subtraction, the ACADVL deficient cell line shows a 93% reduction in the level of the ACADVL protein, the ACADM deficient line shows a 57% reduction in the level of the ACADM protein and the LCHAD deficient cell line shows a 50% reduction of in the level of the HADHA protein. The deficient cell lines characterized protein changes are: (1) ACADVL:p.[N122D], (2) ACADM:p[K304E and (3) HADHB:p[R61H];[R247H].
Protocols
References for Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)
ab118183 has not yet been referenced specifically in any publications.
Publishing research using ab118183? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"