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Product name
Fatty Acid Oxidation Human In-Cell ELISA Kit
Tests
1 x 96 well plate
Sample type
Adherent cells, Suspension cells
Product overview
ab118182 uses quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.
Notes
Upon receipt spin down the contents of the IRDye®-labeled Secondary Antibody tube and protect from light. Store all components upright at 4C. This kit is stable for at least 6 months from receipt.
Tested applications
In-Cell ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 1000X IRDye 800-labeled Secondary antibody | 1 x 0.036ml | |
| 100X ACADM Primary Antibody | 1 x 0.12ml | |
| 100X ACADVL Primary Antibody | 1 x 0.12ml | |
| 100X HADHA Primary Antibody | 1 x 0.12ml | |
| 10X Blocking Buffer | 1 x 20ml | |
| 10x Phosphate buffered Saline | P/N 8209706 | 1 x 100ml |
| 1X Janus Green Stain | 1 x 17ml | |
| 33x Triton X-100 | 1 x 5ml | |
| 400x Tween-20 | P/N 8209708 | 1 x 4ml |
Research areas
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Alternative names
- MS975
Applications
Show applications key
Our Abpromise guarantee covers the use of ab118182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
In-Cell ELISA
Fatty Acid Oxidation Human In-Cell ELISA Kit images:
Immunocytochemistry - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
Antibody specificity demonstrated by immunocytochemistry. Visualization under the microscope was carried out with a completely opened aperture and a very narrow field of visualization at 40x. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab. Note that although LCHAD#1 deficient had a characterized homozygous mutation, the antibody shows a mosaic pattern of staining with about 1/3 of the cells lacking HADHA staining and an overall 40% reduction of signal as observed by ICE.
In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
Panel B shows levels of ACADM protein in two deficient cell lines (ACADM:p. [K304E]).
In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
Panel C shows levels of HADHA protein in two deficient cell lines: (1) HADHA:p.[E474Q] and (2) HADHB:p. [R61H];[R247H]. Note that although LCHAD#1 deficient has a partial deficiency as observed by ICC.
In-Cell ELISA - Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
Panel A shows levels of ACADVL protein in a deficient cell line (ACADVL:p.[N122D]).
Protocols
References for Fatty Acid Oxidation Human In-Cell ELISA Kit (ab118182)
ab118182 has not yet been referenced specifically in any publications.
Publishing research using ab118182? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"