![Western blot - FbxL4 antibody [2352C1a] (ab53541) image](#flx.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> F Box E3 Ligase
Overview
Product name
Anti-FbxL4 antibody [2352C1a]
See all FbxL4 products (2) ...
Description
Mouse monoclonal [2352C1a] to FbxL4
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment of FbxL4 (Human)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum
Clonality
Monoclonal
Clone number
2352C1a
Isotype
IgG1
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> F Box E3 Ligase
-
Western blot - FbxL4 antibody [2352C1a] (ab53541)
![Western blot - FbxL4 antibody [2352C1a] (ab53541) image](/ps/datasheet/Images/53/ab53541/flx.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.
Dot: Use at an assay dependent dilution.
Target
Tissue specificity
Expressed in heart, kidney, liver, lung, pancreas, and placenta, but not in skeletal muscle.
Sequence similarities
Contains 1 F-box domain.
Contains 9 LRR (leucine-rich) repeats.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ9UKA2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- F box and leucine rich repeat protein 4 antibody
- F box protein FBL4/FBL5 antibody
- F box/LRR repeat protein 4 antibody
see all
Database links
- Entrez Gene: 26235 Human
- Omim: 605654 Human
- SwissProt: Q9UKA2 Human
- Unigene: 49526 Human
- Unigene: 728297 Human
Anti-FbxL4 antibody [2352C1a] images:
Western blot - FbxL4 antibody [2352C1a] (ab53541)
Anti-FbxL4 antibody [2352C1a] (ab53541) + Immunized recombinant protein
Predicted band size : 70 kDa
Observed band size : 31 kDa (why is the actual band size different from the predicted?)
The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen
References for Anti-FbxL4 antibody [2352C1a] (ab53541)
ab53541 has not yet been referenced specifically in any publications.
Publishing research using ab53541? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"