Anti-Fc epsilon RI antibody [0.T.195] (ab33568)

Overview

• Product nameAnti-Fc epsilon RI antibody [0.T.195]See all Fc epsilon RI primary antibodies ...
• Description
  Mouse monoclonal [0.T.195] to Fc epsilon RI
• SpecificityThis antibody recognizes Fc epsilon RI.
• Tested applicationsICC/IF, Flow Cyt, IP more details
• Species reactivity
  Reacts with: Rat
  
• Immunogen

  Whole rat basophilic leukemia cells, positive for the rat high affinity receptor recognizing immunoglobulin E (Fc epsilon RI).

• Positive controlBiotinylated rat basophilic leukemia cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number0.T.195
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgE

  • Immunology
  • Immunoglobulins
  • Receptors

Applications

Target

• FunctionBinds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
• Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
• Cellular localizationCell membrane.

Target information above from: UniProt accession P12319 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 25047 Rat
  • SwissProt: P12371 Rat
  • Unigene: 9677 Rat

• Alternative names
  Fc epsilon RI alpha antibodyFc epsilon RI alpha chain antibodyFc epsilon RI alpha-chain antibody

  Fc fragment of IgE high affinity I receptor for alpha polypeptide antibodyFc fragment of IgE, high affinity I, receptor for, alpha subunit antibodyFc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibodyFc IgE receptor alpha polypeptide antibodyFc IgE receptor, alpha chain antibodyFc IgE receptor, alpha polypeptide antibodyFc of IgE high affinity I receptor for alpha polypeptide antibodyFc-epsilon RI-alpha antibodyFCE 1A antibodyFCE1A antibodyFCER 1A antibodyFCER1A antibodyFCERA_HUMAN antibodyFceRI alpha antibodyFcERI antibodyhigh affinity IgE receptor antibodyHigh affinity immunoglobulin epsilon receptor alpha subunit antibodyhigh affinity immunoglobulin epsilon receptor alpha-subunit antibodyHigh affinity immunoglobulin epsilon receptor subunit alpha antibodyIgE Fc receptor alpha subunit antibodyIgE Fc receptor subunit alpha antibodyImmunoglobulin E receptor high affinity of mast cells alpha polypeptide antibodyimmunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody

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Anti-Fc epsilon RI antibody [0.T.195] images

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  Immunocytochemistry/ Immunofluorescence - Fc epsilon RI antibody [0.T.195] (ab33568)This image is courtesy of an Abreview submitted by Nurit Pereg-Azouz.
  ab33568 staining Fc epsilon RI (red) in Rat mast cells (RBL) by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.15 Triton X-100 prior to blocking in 1% BSA and 1% FBS for 30 minutes at 22°C. The primary antibody was diluted 1/500 in PBS with 1% BSA and 1% FBS and incubated with the sample for 1 hour at 22°C. The secondary antibody was Alexa Fluor® 647-conjugated Goat anti-Mouse polyclonal, diluted 1/400.

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