Anti-Fc epsilon RI antibody [CRA1] (Biotin) (ab122882)
- Product nameAnti-Fc epsilon RI antibody [CRA1] (Biotin)See all Fc epsilon RI primary antibodies ...
- DescriptionMouse monoclonal [CRA1] to Fc epsilon RI (Biotin)
- Conjugation notes[biotin]/[IgG] = 8.9
- Specificityab122882 reacts with the Fc epsilon RI subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete with IgE for the receptor binding. Since clone CRA2 (ab122891; ab122915) reacts with the IgE binding site on Fc epsilon RI, it competes with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound Fc epsilon RI.
- Tested applicationsWB, Flow Cyt, ICC/IF more details
- Species reactivityReacts with: Human
Recombinant fragment: MAPAMESPTL LCVALLFFAP DGVLAVPQKP KVSLNPPWNR IFKGENVTLT CNGNNFFEVS STKWFHNGSL SEETNSSLNI VNAK, corresponding to amino acids 1-84 of Human Fc epsilon RI
- Epitopecorresponds to Amino acids 1-84 of Human Fc epsilon RI
- Positive controlCHO/alpha beta gamma cells
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.40
Constituents: 49% PBS, 50% Glycerol
- Concentration information loading...
- PurityIgG fraction
- Purification notesThe IgG fraction was purified from serum free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions. Filter sterilized.
- Clonality Monoclonal
- Clone numberCRA1
- Light chain typekappa
- Research Areas
Our Abpromise guarantee covers the use of ab122882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.|
|Flow Cyt||Flow Cyt: Use at an assay dependent dilution.|
|ICC/IF||ICC/IF: Use at an assay dependent dilution.|
- FunctionBinds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
- Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
- Cellular localizationCell membrane.
- Fc epsilon RI alpha antibodyFc epsilon RI alpha chain antibodyFc epsilon RI alpha-chain antibody
- Fc fragment of IgE high affinity I receptor for alpha polypeptide antibodyFc fragment of IgE, high affinity I, receptor for, alpha subunit antibodyFc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibodyFc IgE receptor alpha polypeptide antibodyFc IgE receptor, alpha chain antibodyFc IgE receptor, alpha polypeptide antibodyFc of IgE high affinity I receptor for alpha polypeptide antibodyFc-epsilon RI-alpha antibodyFCE 1A antibodyFCE1A antibodyFCER 1A antibodyFcer1a antibodyFCERA_HUMAN antibodyFceRI alpha antibodyFcERI antibodyhigh affinity IgE receptor antibodyHigh affinity immunoglobulin epsilon receptor alpha subunit antibodyhigh affinity immunoglobulin epsilon receptor alpha-subunit antibodyHigh affinity immunoglobulin epsilon receptor subunit alpha antibodyIgE Fc receptor alpha subunit antibodyIgE Fc receptor subunit alpha antibodyImmunoglobulin E receptor high affinity of mast cells alpha polypeptide antibodyimmunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody
Anti-Fc epsilon RI antibody [CRA1] (Biotin) images
FACS analysis of CHO/alpha beta gamma cells (1x105) with CRA1 and CRA2 antibodies by indirect-immunostaining, using FITC-labeled secondary antibody.
References for Anti-Fc epsilon RI antibody [CRA1] (Biotin) (ab122882)
This product has been referenced in:
- Untersmayr E et al. The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. PLoS One 5:e9023 (2010). Read more (PubMed: 20126404) »
- Takai T et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). Read more (PubMed: 11272849) »