Anti-Fc epsilon RI antibody [CRA2] (FITC) (ab122891)
- Product nameAnti-Fc epsilon RI antibody [CRA2] (FITC)See all Fc epsilon RI primary antibodies ...
- DescriptionMouse monoclonal [CRA2] to Fc epsilon RI (FITC)
- Conjugation notes[FITC]/[IgG] 8.9
- Specificityab122891 reacts with the Fc epsilon RI subunit on a region that overlaps the region of the IgE binding site, thus it competes with IgE for the receptor binding. Since the clone CRA1 monoclonal antibody reacts with the site different from the IgE binding site on Fc epsilon RI, it does not compete with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound Fc epsilon RI.
- Tested applicationsICC, Flow Cyt, Inhibition Assay more details
- Species reactivityReacts with: Human
Recombinant fragment: FEDSGEYKCQ HQQVNESEPV YLEVFSDWLL LQASAEVVME GQPLFLRCHG WRNWDVYKVI YYKDGEALKY WYENHNISIT NATVEDSG, corresponding to amino acids 85-172 of Human Fc epsilon RI
- EpitopeAmino acids 85-172 of Fc epsilon RI
- Positive controlCHO/alpha beta gamma cells
- Storage instructionsStore at -20°C. Avoid exposure to light.
- Storage bufferpH: 7.40
Constituents: 49% PBS, 50% Glycerol
- Concentration information loading...
- PurityIgG fraction
- Purification notesab122891 was purified from serum free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions. Filter sterilized
- Clonality Monoclonal
- Clone numberCRA2
- Light chain typekappa
- Research Areas
Our Abpromise guarantee covers the use of ab122891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||ICC: Use at an assay dependent dilution.|
|Flow Cyt||Flow Cyt: Use at an assay dependent dilution.|
|Inhibition Assay||Inhib: Use at an assay dependent dilution. Inhibition of binding of IgE with Fc epsilon RI|
- FunctionBinds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
- Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
- Cellular localizationCell membrane.
- Fc epsilon RI alpha antibodyFc epsilon RI alpha chain antibodyFc epsilon RI alpha-chain antibody
- Fc fragment of IgE high affinity I receptor for alpha polypeptide antibodyFc fragment of IgE, high affinity I, receptor for, alpha subunit antibodyFc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibodyFc IgE receptor alpha polypeptide antibodyFc IgE receptor, alpha chain antibodyFc IgE receptor, alpha polypeptide antibodyFc of IgE high affinity I receptor for alpha polypeptide antibodyFc-epsilon RI-alpha antibodyFCE 1A antibodyFCE1A antibodyFCER 1A antibodyFcer1a antibodyFCERA_HUMAN antibodyFceRI alpha antibodyFcERI antibodyhigh affinity IgE receptor antibodyHigh affinity immunoglobulin epsilon receptor alpha subunit antibodyhigh affinity immunoglobulin epsilon receptor alpha-subunit antibodyHigh affinity immunoglobulin epsilon receptor subunit alpha antibodyIgE Fc receptor alpha subunit antibodyIgE Fc receptor subunit alpha antibodyImmunoglobulin E receptor high affinity of mast cells alpha polypeptide antibodyimmunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody
Anti-Fc epsilon RI antibody [CRA2] (FITC) images
FACS analysis of CHO/alpha beta gamma cells (1x105) with CRA1 and CRA2 antibodies by indirect-immunostaining using FITC-labeled secondary antibody.
References for Anti-Fc epsilon RI antibody [CRA2] (FITC) (ab122891)
This product has been referenced in:
- Takai T et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). Read more (PubMed: 11272849) »
- Goto T et al. Enhanced expression of the high-affinity receptor for IgE (Fc(epsilon)RI) associated with decreased numbers of Langerhans cells in the lesional epidermis of atopic dermatitis. J Dermatol Sci 27:156-61 (2001). Read more (PubMed: 11641054) »