Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab34362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 10 kDa.
IP Use at an assay dependent dilution.

Target

  • FunctionBinds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
  • Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
  • Cellular localizationCell membrane.
  • Target information above from: UniProt accession P12319 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Fc epsilon RI alpha antibody
    • Fc epsilon RI alpha chain antibody
    • Fc epsilon RI alpha-chain antibody
    • Fc fragment of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc fragment of IgE, high affinity I, receptor for, alpha subunit antibody
    • Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibody
    • Fc IgE receptor alpha polypeptide antibody
    • Fc IgE receptor, alpha chain antibody
    • Fc IgE receptor, alpha polypeptide antibody
    • Fc of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc-epsilon RI-alpha antibody
    • FCE 1A antibody
    • FCE1A antibody
    • FCER 1A antibody
    • FCER 1A antibody
    • FCER1A antibody
    • FCERA_HUMAN antibody
    • FceRI alpha antibody
    • FcERI antibody
    • high affinity IgE receptor antibody
    • High affinity immunoglobulin epsilon receptor alpha subunit antibody
    • high affinity immunoglobulin epsilon receptor alpha-subunit antibody
    • High affinity immunoglobulin epsilon receptor subunit alpha antibody
    • IgE Fc receptor alpha subunit antibody
    • IgE Fc receptor alpha subunit antibody
    • IgE Fc receptor subunit alpha antibody
    • Immunoglobulin E receptor high affinity of mast cells alpha polypeptide antibody
    • immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody
    see all

Anti-Fc epsilon RI antibody images

  • All lanes : Anti-Fc epsilon RI antibody (ab34362)

    Lane 1 : Lysates prepared from Caco2/TC7 subconfluent cell line.
    Lane 2 : Lysates prepared from Caco2/TC7 confluent cell line.
    Lane 3 : Lysates prepared from HCT8 subconfluent cell line.
    Lane 4 : Lysates prepared from HCT8 confluent cell line.
    Lane 5 : Lysates prepared from RBL transfected cells.


    Predicted band size : 10 kDa

    Image from Untersmayr E et al, PLoS One. 2010 Feb 2;5(2):e9023, Fig 8.

References for Anti-Fc epsilon RI antibody (ab34362)

This product has been referenced in:
  • Untersmayr E  et al. The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. PLoS One 5:e9023 (2010). WB ; Human . Read more (PubMed: 20126404) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (lung)
Loading amount 25 µg
Specification lung
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted May 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"