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Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)

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Overview

Product name

Anti-Ferritin Heavy Chain antibody [EPR3005Y]
See all Ferritin Heavy Chain products (7) ...

Description

Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain

Specificity

This antibody recognizes only the Ferritin heavy chain.

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues near the C terminal of human Ferritin Heavy Chain

Positive control

Human liver, SH-SY5Y, human brain and HeLa cell lysates and human kidney tissue

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR3005Y

Isotype

IgG

  • Western blot - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)Western blot - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/2000.Detec...Read more →

    WB: 1/500 - 1/2000.Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.Per...Read more →

    IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Application notes

Is unsuitable for ,Flow Cyt,ICC/IF or IP.

Target

Relevance

Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy; 21 kDa) and L (light; 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.

Cellular localization

Cytoplasmic

Alternative names

  • Apoferritin antibody
  • Cell proliferation inducing gene 15 protein antibody
  • F HC antibody
  • Ferritin H subunit antibody
  • Ferritin heavy chain antibody
  • Ferritin heavy polypeptide 1 antibody
  • FHC antibody
  • FRIH antibody
  • FTH 1 antibody
  • FTH antibody
  • FTH1 antibody
  • FTH1 protein antibody
  • FTHL 6 antibody
  • FTHL6 antibody
  • Iron overload autosomal dominant antibody
  • MGC104426 antibody
  • PIG 15 antibody
  • PIG15 antibody
  • Placenta immunoregulatory factor antibody
  • PLIF antibody
  • Proliferation inducing gene 15 protein antibody
  • Proliferation inducing protein 15 antibody
see all

Database links

Anti-Ferritin Heavy Chain antibody [EPR3005Y] images:

  Western blot - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)

Western blot - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)

Lanes 1 - 3 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/500 dilution
Lane 4 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution
Lane 5 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution

Lane 1 : Human liver lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : Human brain lysate
Lane 4 : Human brain lysate
Lane 5 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 21 kDa
Observed band size : 21 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)

ab75972 at 1/100 dilution staining Ferritin Heavy Chain in human kidney tissue, using a HRP/AP polymerized secondary antibody.

References for Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)

ab75972 has not yet been referenced specifically in any publications.

Publishing research using ab75972? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"