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Western blot - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
![Western blot - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](/ps/datasheet/images/ab75972_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](/ps/datasheet/images/ab75972_2.jpg)
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Product Name
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Ferritin Heavy Chain antibody [EPR3005Y]
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See all Ferritin Heavy Chain antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain
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Immunogen
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A synthetic peptide corresponding to residues near the C terminal of human Ferritin Heavy Chain
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Specificity
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This antibody recognizes only the Ferritin heavy chain.
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. WB: 1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Is unsuitable for Flow Cyt, ICC/IF or IP.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver, SH-SY5Y, human brain and HeLa cell lysates and human kidney tissue
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Metabolism >> Vitamins / Minerals Cardiovascular >> Blood >> Serum Proteins Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Relevance
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Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy; 21 kDa) and L (light; 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EPR3005Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ferritin Heavy Chain antibody [EPR3005Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Ferritin Heavy Chain antibody [EPR3005Y] images, references, products related to ab75972 and other tools.
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Ferritin Heavy Chain antibody [EPR3005Y] images:
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Western blot - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
Lanes 1 - 3 : Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/500 dilution Lane 4 : Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution Lane 5 : Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution
Lane 1 : Human liver lysate Lane 2 : SH-SY5Y lysate Lane 3 : Human brain lysate Lane 4 : Human brain lysate Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 21 kDa Observed band size : 21 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)
ab75972 at 1/100 dilution staining Ferritin Heavy Chain in human kidney tissue, using a HRP/AP polymerized secondary antibody.
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Search PubMed (MEDLINE) for references to Ferritin Heavy Chain
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Ferritin Heavy Chain antibody [EPR3005Y] - more information
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Ferritin Heavy Chain antibody [EPR3005Y] - related products:
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Ferritin Heavy Chain antibody [EPR3005Y] - other tools:
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