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ELISA - Ferritin Heavy Chain antibody (ab36660)
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Product Name
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Ferritin Heavy Chain antibody
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See all Ferritin Heavy Chain antibodies (7)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to Ferritin Heavy Chain
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Immunogen
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Synthetic peptide, corresponding to amino acids 1-182 of Human Ferritin Heavy Chain
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 1 µg/ml.
WB: Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ELISA: Purified Human Ferritin protein.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Metabolism >> Vitamins / Minerals
Cardiovascular >> Blood >> Serum Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Relevance
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Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy; 21 kDa) and L (light; 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ferritin Heavy Chain antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Ferritin Heavy Chain antibody images:
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 ELISA - Ferritin Heavy Chain antibody (ab36660)
Ab36660, at a fixed concentration of 1 µg/ml, staining Ferritin heavy chain from purified Ferritin protein by ELISA. Goat anti IgY HRP was the secondary antibody. A serial dilution of the antigen was used.
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References for Ferritin Heavy Chain antibody (ab36660)
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This product has been used in:
Barbeito AG et al. Abnormal iron metabolism and oxidative stress in mice expressing a mutant form of the ferritin light polypeptide gene. J Neurochem 109:1067-78 (2009). WB; Mouse. PubMed: 19519778
If you publish research using ab36660 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Ferritin Heavy Chain
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