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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-Ferritin Heavy Chain antibody
See all Ferritin Heavy Chain products (7) ...
Description
Chicken polyclonal to Ferritin Heavy Chain
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide, corresponding to amino acids 1-182 of Human Ferritin Heavy Chain
Positive control
ELISA: Purified Human Ferritin protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Signal Transduction >> Metabolism >> Vitamins / Minerals
Cardiovascular >> Blood >> Serum Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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ELISA - Ferritin Heavy Chain antibody (ab36660)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab36660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
ELISA: Use a concentration of 1 µg/ml
Target
Relevance
Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy; 21 kDa) and L (light; 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.
Cellular localization
Cytoplasmic
Alternative names
- Apoferritin antibody
- Cell proliferation inducing gene 15 protein antibody
- F HC antibody
see all
Database links
- Entrez Gene: 2495 Human
- Entrez Gene: 14319 Mouse
- Omim: 134770 Human
- SwissProt: P02794 Human
- SwissProt: P09528 Mouse
Anti-Ferritin Heavy Chain antibody images:
ELISA - Ferritin Heavy Chain antibody (ab36660)
Ab36660, at a fixed concentration of 1 µg/ml, staining Ferritin heavy chain from purified Ferritin protein by ELISA. Goat anti IgY HRP was the secondary antibody. A serial dilution of the antigen was used.
References for Anti-Ferritin Heavy Chain antibody (ab36660)
This product has been referenced in:
- Barbeito AGet al. Abnormal iron metabolism and oxidative stress in mice expressing a mutant form of the ferritin light polypeptide gene. J Neurochem 109:1067-78 (2009). WB; Mouse.Read more (PubMed: 19519778) »
See 1 publication for this product
Publishing research using ab36660? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"