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Anti-Ferritin Heavy Chain antibody
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Chicken polyclonal to Ferritin Heavy Chain
WB, ELISAmore details
Reacts with
Mouse, Human
Synthetic peptide, corresponding to amino acids 1-182 of Human Ferritin Heavy Chain
ELISA: Purified Human Ferritin protein.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: PBS
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Immunogen affinity purified
Polyclonal
IgY
Signal Transduction >> Metabolism >> Vitamins / Minerals
Cardiovascular >> Blood >> Serum Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
ELISA - Ferritin Heavy Chain antibody (ab36660)
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Our Abpromise guarantee covers the use of ab36660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
ELISA: Use a concentration of 1 µg/ml
Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy; 21 kDa) and L (light; 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.
Cytoplasmic
ELISA - Ferritin Heavy Chain antibody (ab36660)

Ab36660, at a fixed concentration of 1 µg/ml, staining Ferritin heavy chain from purified Ferritin protein by ELISA. Goat anti IgY HRP was the secondary antibody. A serial dilution of the antigen was used.
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