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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Ferritin Heavy Chain protein (Human)
See all Ferritin Heavy Chain products (2) ...
Protein description
Recombinant full length human Ferritin Heavy Chain; 183 amino acids, 21 kDa. Swiss ID = P02794.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Purification notes
ab78877is purified using conventional chromatography techniques.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10% Glycerol, 20mM Tris, 1mM DTT, pH 7.5
Concentration
Concentration information loading...
Sequence notes
MTTASTSQVR QNYHQDSEAA INRQINLELY ASYVYLSMSY YFDRDDVALK NFAKYFLHQS HEEREHAEKL MKLQNQRGGR IFLQDIKKPD CDDWESGLNA MECALHLEKN VNQSLLELHK LATDKNDPHL CDFIETHYLN EQVKAIKELG DHVTNLRKMG APESGLAEYL FDKHTLGDSD NES
Research areas
Signal Transduction >> Metabolism >> Vitamins / Minerals
Cardiovascular >> Blood >> Serum Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
WB: Use at an assay dependent concentration. (Ab78877 can be used as a WB positive control in conjunction with ab65080. )
Protein info
Relevance
Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy; 21 kDa) and L (light; 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.
Cellular localization
Cytoplasmic
Ferritin Heavy Chain protein (Human) images:
Western blot - Anti-Ferritin Heavy Chain antibody (ab65080)
All lanes : Anti-Ferritin Heavy Chain antibody (ab65080) at 1 µg/ml
Lane 1 : Ferritin Heavy Chain protein (Human) (ab78877) at 0.1 µg
Lane 2 : Ferritin Heavy Chain protein (Human) (ab78877) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
SDS-PAGE - Ferritin Heavy Chain protein (Human) (ab78877)
15% SDS-PAGE showing ab78877 at approximately 21kDa (3µg).
References for Ferritin Heavy Chain protein (Human) (ab78877)
ab78877 has not yet been referenced specifically in any publications.
Publishing research using ab78877? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"