Overview
- Product nameFetuin A Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 10 pg/ml
- Range312 pg/ml - 20000 pg/ml
- Species reactivityReacts with: Human
- Product overview
ab119594 Fetuin A Human ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Fetuin A specific-specific polyclonal antibodies were precoated onto 96-well plates. The Human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Fetuin A amount of sample captured in plate.
No detectable cross-reactivity with any other protein.
- Notes
Store at 2-8°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96-well plate precoated with anti-Human Fetuin A antibody (12 strips) 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-Human Fetuin A antibody 1 x 130µl Lyophilized recombinant Human Fetuin A standard 2 x 20ng Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml -
Research Areas
Contains 2 cystatin fetuin-A-type domains.
modificationsPhosphorylation sites are present in the extracelllular medium.
Target information above from: UniProt accession
P02765
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A2HSAHSAhsg
- Alpha 2 HS glycoproteinAlpha-2-HS-glycoprotein chain BAlpha-2-Z-globulinBa-alpha-2-glycoproteinFETUAFETUA_HUMANFetuin-AHSGA
see all
- Entrez Gene: 197 Human
- Omim: 138680 Human
- SwissProt: P02765 Human
- Unigene: 324746 Human
Applications
Our Abpromise guarantee covers the use of ab119594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Fetuin A Human ELISA Kit images
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Sandwich ELISA - Fetuin A Human ELISA Kit (ab119594)Representative standard curve using ab119594.
Protocols
References for Fetuin A Human ELISA Kit (ab119594)
ab119594 has not yet been referenced specifically in any publications.
