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Anti-Fibrillarin antibody [28F2] - Nucleolar Marker (ab4575)

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Overview

Product name

Anti-Fibrillarin antibody [28F2] - Nucleolar Marker
See all Fibrillarin products (5) ...

Description

Mouse monoclonal [28F2] to Fibrillarin - Nucleolar Marker

Tested applications

WB, IF, IPmore details

Cross reactivity

Reacts with

Saccharomyces cerevisiae

Immunogen

Nuclear preparation (S. cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.

General notes

Pfam number: PF01269

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 10mM Sodium Azide
Constituents: Tissue Culture Supernatant

Purity

Tissue culture supernatant

Purification notes

Sterile filtered.

Clonality

Monoclonal

Clone number

28F2

Isotype

IgG3

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nucleolus

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

  • IF

     

  • IP

     

Application notes

IF: 1/2000 - 1/20000 (yeast cells).
IP: Use 2-4 µl for an amount of lysate derived from 5 OD600 units of yeast culture.
WB: 1/10000 (cell lysates)- 1/25000 (nuclear fractions)(yeast protein samples). Predicted molecular weight: 34 kDa. For other (non-ECL) western detection methods, 1/1000 - 1/5000.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.

Sequence similarities

Belongs to the methyltransferase superfamily. Fibrillarin family.

Post-translational
modifications

By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

Cellular localization

Nucleus > nucleolus. Fibrillar region of the nucleolus.

Target information above from: UniProt accessionP22087 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 34 kD nucleolar scleroderma antigen antibody
  • 34 kDa nucleolar scleroderma antigen antibody
  • FBL antibody
  • FBRL_HUMAN antibody
  • FIB antibody
  • FIB1 antibody
  • Fibrillarin antibody
  • FLRN antibody
  • Nop1p antibody
  • RNU3IP1 antibody
  • rRNA 2' O methyltransferase fibrillarin antibody
  • rRNA 2'-O-methyltransferase fibrillarin antibody
see all

References for Anti-Fibrillarin antibody [28F2] - Nucleolar Marker (ab4575)

This product has been referenced in:

  • Merl Jet al. Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes. Nucleic Acids Res : (2010). ICC/IF.Read more (PubMed: 20100801) »

See 1 publication for this product

Publishing research using ab4575? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"