Fibrinogen Mouse ELISA Kit (ab108843)
- Product nameFibrinogen Mouse ELISA KitSee all Fibrinogen kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Urine
- Assay typeSandwich
- Sensitivity= 3 ng/ml
- Range3.125 ng/ml - 200 ng/ml
- Assay time3h 30m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
Fibrinogen (Fibrinogen) is a homodimer of molecular mass 340 kDa, made up of two sets of alpha, beata and gamma polypeptide chains, and synthesized in the parenchymal cell of the hepatocyte and in the megakaryocyte. Fibrinogen plays a major role in coagulation, and both elevated and decreased levels have clinical significance. Upon cleavage by thrombin in the initial stages of coagulation activation, Fibrinogen self-assembles to yield a fibrin clot matrix that subsequently is crosslinked by factor XIIIa to form an insoluble network. Fibrinogen also binds to the platelet glycoprotein IIbIIIa receptor so as to form bridges between platelets, thus facilitating aggregation. Elevated plasma Fibrinogen has been identified as an independent risk factor for coronary atherosclerosis and ischemic heart disease. Individuals with congenital absence of Fibrinogen, termed afibrinogenemia, have prolonged bleeding times.
ab108843 Fibrinogen Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of mouse Fibrinogen in cell culture media, and urine samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures mouse Fibrinogen in 3.5 hours. A polyclonal antibody specific for mouse Fibrinogen has been pre-coated onto a 96-well microplate with removable strips. Fibrinogen in standards and samples is sandwiched by the immobilized polyclonal antibody and biotinylated polyclonal antibody specific for mouse Fibrinogen, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Mouse Fibrinogen Antibody (25x) 1 x 270µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Fibrinogen Microplate (12 strips of 8 wells) 1 unit Fibrinogen Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 15ml
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
modificationsThe alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium.
- FGAFIBA_HUMANFibrinogen alpha chain
Our Abpromise guarantee covers the use of ab108843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Fibrinogen Mouse ELISA Kit (ab108843)
ab108843 has not yet been referenced specifically in any publications.