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Overview

  • Product nameFibrinogen Mouse ELISA KitSee all Fibrinogen kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine
  • Assay typeSandwich
  • Sensitivity
    = 3 ng/ml
  • Range
    3.125 ng/ml - 200 ng/ml
  • Recovery

    98 %

  • Assay time
    3h 30m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Fibrinogen (Fibrinogen) is a homodimer of molecular mass 340 kDa, made up of two sets of alpha, beata and gamma polypeptide chains, and synthesized in the parenchymal cell of the hepatocyte and in the megakaryocyte. Fibrinogen plays a major role in coagulation, and both elevated and decreased levels have clinical significance. Upon cleavage by thrombin in the initial stages of coagulation activation, Fibrinogen self-assembles to yield a fibrin clot matrix that subsequently is crosslinked by factor XIIIa to form an insoluble network. Fibrinogen also binds to the platelet glycoprotein IIbIIIa receptor so as to form bridges between platelets, thus facilitating aggregation. Elevated plasma Fibrinogen has been identified as an independent risk factor for coronary atherosclerosis and ischemic heart disease. Individuals with congenital absence of Fibrinogen, termed afibrinogenemia, have prolonged bleeding times.

    ab108843 Fibrinogen Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of mouse Fibrinogen in cell culture media, and urine samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures mouse Fibrinogen in 3.5 hours. A polyclonal antibody specific for mouse Fibrinogen has been pre-coated onto a 96-well microplate with removable strips. Fibrinogen in standards and samples is sandwiched by the immobilized polyclonal antibody and biotinylated polyclonal antibody specific for mouse Fibrinogen, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionFibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • DomainA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        FGAFIBA_HUMANFibrinogen alpha chain
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108843 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Fibrinogen Mouse ELISA Kit images

      • Sandwich ELISA - Fibrinogen Mouse ELISA Kit 1 x 96 Well Plate (ab108843)
        Sandwich ELISA - Fibrinogen Mouse ELISA Kit 1 x 96 Well Plate (ab108843)

      Protocols

      References for Fibrinogen Mouse ELISA Kit (ab108843)

      ab108843 has not yet been referenced specifically in any publications.

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      What part of the fibrinogen protein does this antibody detect? I am looking for an antibody that is specific to the gamma chain of fibrinogen.

      This kit is not specific for the gamma chain; however, if you wanted to create your own ELISA assay we do have several antibodies that are specific to the gamma chain of fibrinogen.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"