Overview

  • Product nameAnti-Fibrinogen antibodySee all Fibrinogen primary antibodies ...
  • Description
    Rabbit polyclonal to Fibrinogen
  • Tested applicationsIHC-P, ICC/IF, ELISA, Functional Studies, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
  • Immunogen

    Purified fibrinogen from human plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab34269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 - 10 µg/ml.
ELISA 1/3000.
Functional Studies Use at an assay dependent dilution. This antibody has been tested in plasma clotting assays. The plasma clotting time was prolonged (but not completely neutralized) by the antibody. Testing has indicated that these neutralisation assays work only when this antibody has been protein A column purified first.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) as Capture.

Target

  • FunctionFibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • DomainA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications
    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P02671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • FGA antibody
    • FGA protein antibody
    • FGB antibody
    • FGG antibody
    • Fib2 antibody
    • FIBA_HUMAN antibody
    • Fibrinogen A alpha polypeptide antibody
    • Fibrinogen A alpha polypeptide chain antibody
    • Fibrinogen alpha chain antibody
    • Fibrinogen B alpha polypeptide antibody
    • Fibrinogen beta chain antibody
    • Fibrinogen G alpha polypeptide antibody
    • Fibrinogen gamma chain antibody
    • fibrinogen, B beta polypeptide antibody
    • fibrinogen, G gamma polypeptide antibody
    • fibrinogen, gamma polypeptide antibody
    • Fibrinogen--alpha -polypeptide chain antibody
    • Fibrinogen--beta -polypeptide chain antibody
    • Fibrinogen--gamma-polypeptide chain antibody
    • MGC104327 antibody
    • MGC119422 antibody
    • MGC119423 antibody
    • MGC119425 antibody
    • MGC120405 antibody
    see all

Anti-Fibrinogen antibody images

  • Standard Curve for Fibrinogen (Analyte: Fibrinogen protein (Human) (ab84410)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) at 5µg/ml and Detector Antibody Rabbit polyclonal to Fibrinogen (ab34269) at 0.5µg/ml.
  • Ab34269 staining human liver. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab34269 staining Fibrinogen in Human liver/placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer, pH 6.0 for 20 minutes at 100°C. The primary antibody was diluted 1/2000 and incubated with the sample for 20 minutes at 25°C. An HRP polymer-conjugated mouse anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of rat heart, staining Fibrinogen with ab34269.

    Tissue was fixed with formaldehyde and blocked with 0.25% BSA for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1.5 µg/ml in 1% BSA in TBS) for 20 minutes. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

Protocols

References for Anti-Fibrinogen antibody (ab34269)

This product has been referenced in:
  • Zhou HF  et al. Fibrinogen-specific antibody induces abdominal aortic aneurysm in mice through complement lectin pathway activation. Proc Natl Acad Sci U S A 110:E4335-44 (2013). ICC/IF ; Mouse . Read more (PubMed: 24167262) »
  • Zhang Y  et al. In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice. PLoS One 7:e30262 (2012). ICC/IF ; Mouse . Read more (PubMed: 22272319) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Heart/Plasma)
Specification Heart/Plasma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Protein Block (Casein) as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

DISCOUNT CODE: ***
Expiration date: February 12th, 2013
Value: $337

I am very pleased to hear you would like to accept our offer and test ab34269 in rat. This code will give you a discount off your next order before the expiration ...

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Thank you for contacting Abcam regarding ab34269.


This antibody is a whole antiserum derivedfrom rabbit, meaning, it has not been purified further. The antibody can be stored in this format in small aliquots at -20oC. The dilution buffer ...

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Thank you for contacting us. The antibody ab34269 may be a good choice for what you describe, though it has not, to our knowledge been tested in exactly that way. The antibody is a whole serum, unpurified. The negative control for this, to account for ...

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Thank you for contacting Abcam.

I have talked to the lab and they have informed me that this antibody will recognize both Fibrinogen and Fibrin.

If there is anything else I can help you with, please let me know.

Thank you for your phone call. I am still waiting for information regarding the immunogens for ab92481 and ab92510, but I will let you know as soon as I have any additional information.

DISCOUNT CODE: ***

Expiration date: August 16t...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver, placenta)
Specification liver, placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6, 20 min, 100C
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Dec 02 2011

Thanks for your reply and for your patience while I have contacted the lab regarding our fibrinogen antibodies. Ab34269 has been successfully tested against fibrin, and it is suitable for staining human formalin fixed paraffin-embedded tissue. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"