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Fibrinopeptide B protein (Tagged-His Tag) (ab91864)

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Overview

  • Product nameFibrinopeptide B protein (Tagged-His Tag)See all Fibrinopeptide B proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 159-488 (330 amino acids) of Human Fibrinopeptide B with N terminal His tag; Predicted MWt 39 kDa (UniProt P02675).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 118 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesQKQVKDNENVVNEYSSELEKHQLYIDETVNSNIPTNLRVL RSILENLR SKIQKLESDVSAQMEYCRTPCTVSCNIPVV SGKECEEIIRKGGETSEM YLIQPDSSVKPYRVYCDMNT ENGGWTVIQNRQDGSVDFGRKWDPYKQG FGNVATNTDG KNYCGLPGEYWLGNDKISQLTRMGPTELLIEMEDWKGD KVKAHYGGFTVQNEANKYQISVNKYRGTAGNALMDGASQL MGENRTMT IHNGMFFSTYDRDNDGWLTSDPRKQCSKED GGGWWYNRCHAANPNGRY YWGGQYTWDMAKHGTDDGVV WMNWKGSWYSMRKMSMKIRPFF
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91864 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        2510049G14RikAb1-181Ab1-216
        Ac1-581FGBFIBB_HUMANFibrinogen B beta polypeptideFibrinogen beta chainFibrinogen beta polypeptide chainFibrinopeptide BLiver regeneration-related protein LRRG036/LRRG043/LRRG189MGC104327MGC120405OTTHUMP00000197066
      see all
  • FunctionFibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Involvement in diseaseDefects in FGB are a cause of thrombophilia.
    Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen.
  • Sequence similaritiesContains 1 fibrinogen C-terminal domain.
  • DomainA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02675 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Fibrinopeptide B protein (Tagged-His Tag) images

    • SDS-PAGE - Fibrinopeptide B protein (Tagged-His Tag) (ab91864)
      SDS-PAGE - Fibrinopeptide B protein (Tagged-His Tag) (ab91864)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Fibrinopeptide B protein (Tagged-His Tag) (ab91864)

    ab91864 has not yet been referenced specifically in any publications.

    Publishing research using ab91864 ? Please let us know so that we can cite the reference in this datasheet

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