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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
Overview
Product name
Anti-Fibroblast activation protein, alpha antibody - Spacer region
See all Fibroblast activation protein, alpha products (6) ...
Description
Rabbit polyclonal to Fibroblast activation protein, alpha - Spacer region
Specificity
This antibody recognizes both long isoforms of Fibroblast activation protein, alpha, but does not recognize other DPP family members.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide of Human Fibroblast activation protein, alpha based on the spacer region.
(Peptide available as ab41275.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
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Western blot - Fibroblast activation protein, alpha antibody - Spacer region (ab28245)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Detects a band of approximately 110-120 kDa due to post translational modifications and reduction. (Predicted molecular weight: 88 kDa). Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
Tissue specificity
Fibroblast specific.
Sequence similarities
Belongs to the peptidase S9B family.
Post-translational
modifications
N-glycosylated.
The N-terminus may be blocked.
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
Target information above from: UniProt accessionQ12884
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 170 kDa melanoma membrane bound gelatinase antibody
- DPPIV antibody
- Integral membrane serine protease antibody
see all
Database links
- Entrez Gene: 2191 Human
- Entrez Gene: 14089 Mouse
- Entrez Gene: 192203 Rat
- Omim: 600403 Human
- SwissProt: Q12884 Human
- SwissProt: P97321 Mouse
- Unigene: 654370 Human
- Unigene: 41816 Mouse
Anti-Fibroblast activation protein, alpha antibody - Spacer region images:
Western blot - Fibroblast activation protein, alpha antibody - Spacer region (ab28245)
All lanes : Anti-Fibroblast activation protein, alpha antibody - Spacer region (ab28245) at 1/1000 dilution
Lane 1 : r-Human FAPalpha at 0.08 µg
Lane 2 : r-Human FAPalpha at 0.04 µg
Lane 3 : r-Human FAPalpha at 0.02 µg
Lane 4 : r-Human FAPalpha at 0.01 µg
Lane 5 : r-Human FAPalpha at 0.005 µg
Predicted band size : 88 kDa
Protocols
References for Anti-Fibroblast activation protein, alpha antibody - Spacer region (ab28245)
This product has been referenced in:
- Sromova Let al. Dipeptidyl peptidase-IV in synovial fluid and in synovial fluid mononuclear cells of patients with rheumatoid arthritis. Clin Chim Acta 411:1046-50 (2010). Flow Cyt; Human.Read more (PubMed: 20361950) »
See 1 publication for this product
Publishing research using ab28245? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"