Anti-Fibroblast activation protein, alpha antibody - Stalk region (ab28244)

Hi, I want to buy Fibroblast activation protein antibody, and I saw there are 4 products on your website. Ab28243 Catalytic domain Ab28246 Catalytic insert Ab28245 Spacer Ab28244 Stalk I want to do immunofluorescence in rat fibroblast to see if my treatment can activate fibroblast or not. I want to use FAP as a marker for the activated fibroblast. So which antibody do you suggest? Thanks!

ANSWER:

Thank you for contacting Abcam.

I would recommend using ab28244 or ab28246 as each of these is guaranteed to work in IF on rat tissues under our Abpromise.

The immunogen for ab28244 Fibroblast activation protein, alpha antibody starts at the beginning of the stalk region.This region starts at amino acid 30 of the 760 amino acid sequence of human FAP-alpha, and follows the transmembrane domain. Cleavage of the stalk region releases the shed form of FAP-alpha.

ab28246(Fibroblast activation protein, alpha antibody - Catalytic insert) will recognize the 759 amino acid sequence of human FAP-alpha which has an insert in the catalytic domain.

These products are covered by our Abpromise guarantee. If you experience any issues with these products we offer scientific support, replacement or refund under our Abpromise guarantee, for a period of 6 months from purchase, if used according to specifications listed on our datasheet.

Thank you for the information. Are there any papers referencing use of this antibody for IHC on MOUSE paraffin-embedded tissue? And what dilution would you recommend

ANSWER:

Thank you for contacting Abcam.
We do not know of any publications which have used this product in IHC-P in mouse tissues.
A good starting point for determining the optimal concentration is to use the dilution used in the earlier referenced paper, 1:600. Of course you will need to titer out the best concentration but using this reference for your experiments will give you an excellent start.
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Regarding ab28244, has this antibody been tested for IHC of fixed/paraffin-embedded mouse tissue, and if so, are you able to provide any sample images and/or recommended dilution/staining conditions? I was not able to find any reference of this antibody in the literature. Thank you.

ANSWER:

Thank you for contacting us.

This product was used in IHC-P in the following publication: Bhati R et al. Molecular characterization of human breast tumor vascular cells. Am J Pathol 172:1381-90 (2008). IHC-P; Human. (PubMed: 18403594) (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2329846/?tool=pubmed)
Where it is referenced as Rabbit polyclonal antibody to fibroblast activation protein (FAP), α-Stalk region (Abcam, Cambridge, MA) and was used at 1:600 dilution.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Hi,

We are looking for an antibody to sort porcine fibroblasts by flow cytometry. Do you have any antibody that may work in pigs? Otherwise, do you offer any kind of testing sample and/or discount to try the antibody inadifferent specie other than those listed in the datasheet?

Thanks for your help,

ANSWER:

Thank you for your enquiry and your interest in our products.

The tested applications and the species are normally listed on the on-line product datasheets. Generally, we test our antibodies in human, mouse and rat samples - very few antibodies are characterized in specifically in porcine. However, it does not necessary mean that they are unsuitable in this species but we have no preliminary data yet to support the cross-reactivity.

I have conducted a search for you and found some antibodies (ab28244, ab44971, ab51824) which recognize fibroblasts and are suitable for Flow cytometry but no cross-reactivity data with porcine so far. We can certainly offer a discount code for testings and the Terms and Conditions of this offer can be found at the following site: http://www.abcam.com/collaborationdiscount.

If you are interested in any particular fibroblast markers, you may wish to conduct a search using our website and check some publications using PubMed websites.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Dear Sirs,

I'm interested in your anti-FAP Ab ab28244. I would like to know the epitope sequence or an indicative aa region recognized by the

Ab.

Thanks for your collaboration.

Best regards

ANSWER:

Thank you for contacting Abcam regarding ab28244.

Unfortunately the immunogen information for ab28244 is considered proprietary and I do not have access to that information.

Please accept my apologies for not being able to provide you with the information you required. If there is anything else I can do help you, please do not hesitate to contact me.