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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
Overview
Product name
Anti-Fibroblast activation protein, alpha antibody - Stalk region
See all Fibroblast activation protein, alpha products (6) ...
Description
Rabbit polyclonal to Fibroblast activation protein, alpha - Stalk region
Specificity
This antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein, alpha but does not recognize other DPP family members.
Tested applications
ICC/IF, IHC-P, Flow Cyt, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide of Human Fibroblast activation protein, alpha, corresponding to the stalk region.
(Peptide available as ab41274.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 10 µg/ml.
IHC-P: Use at an assay dependent dilution.
Flow Cyt: 1/50(PubMed: 20361950)
WB: 1/1000 - 1/5000.Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa).(1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.)
Target
Function
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
Tissue specificity
Fibroblast specific.
Sequence similarities
Belongs to the peptidase S9B family.
Post-translational
modifications
N-glycosylated.
The N-terminus may be blocked.
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
Target information above from: UniProt accessionQ12884
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 170 kDa melanoma membrane bound gelatinase antibody
- DPPIV antibody
- Integral membrane serine protease antibody
see all
Database links
- Entrez Gene: 2191 Human
- Entrez Gene: 14089 Mouse
- Entrez Gene: 192203 Rat
- Omim: 600403 Human
- SwissProt: Q12884 Human
- SwissProt: P97321 Mouse
- Unigene: 654370 Human
- Unigene: 41816 Mouse
Anti-Fibroblast activation protein, alpha antibody - Stalk region images:
Western blot - Fibroblast activation protein, alpha antibody - Stalk region (ab28244)
All lanes : Anti-Fibroblast activation protein, alpha antibody - Stalk region (ab28244) at 1/1000 dilution
Lane 1 : Recombinant Human FAPalpha at 0.08 µg
Lane 2 : Recombinant Human FAPalpha at 0.04 µg
Lane 3 : Recombinant Human FAPalpha at 0.02 µg
Lane 4 : Recombinant Human FAPalpha at 0.01 µg
Lane 5 : Recombinant Human FAPalpha at 0.005 µg
Predicted band size : 88 kDa
Immunocytochemistry/ Immunofluorescence - Fibroblast activation protein, alpha antibody - Stalk region (ab28244)
ICC/IF image of ab28244 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28244, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Fibroblast activation protein, alpha antibody - Stalk region (ab28244)
This product has been referenced in:
- Sromova Let al. Dipeptidyl peptidase-IV in synovial fluid and in synovial fluid mononuclear cells of patients with rheumatoid arthritis. Clin Chim Acta 411:1046-50 (2010). Flow Cyt; Human.Read more (PubMed: 20361950) »
- Bhati Ret al. Molecular characterization of human breast tumor vascular cells. Am J Pathol 172:1381-90 (2008). IHC-P; Human.Read more (PubMed: 18403594) »
See all 2 publications for this product
Publishing research using ab28244? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"