|
|
|
|
Western blot - Fibroblast activation protein, alpha antibody - Stalk region (ab28244)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence-Fibroblast activation protein, alpha antibody - Stalk region(ab28244)
(enlarge)
|
|
|
|
|
Product Name
|
Fibroblast activation protein, alpha antibody - Stalk region
|
| |
See all Fibroblast activation protein, alpha antibodies (6)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Fibroblast activation protein, alpha - Stalk region
|
|
Immunogen
|
Synthetic peptide of Human Fibroblast activation protein, alpha, corresponding to the stalk region.
(Peptide available as ab41274.)
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
This antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein, alpha but does not recognize other DPP family members.
We have a range of domain specific antibodies for this target. For a full list please see all Fibroblast activation protein, alpha antibodies
|
|
Tested applications
(see key)
|
ICC/IF, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 1 - 10 µg/ml.
IHC-P: Use at an assay dependent dilution (PMID 18403594).
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cell Membrane; single-pass type II membrane protein. Found in cell surface lamellipodia, invadopodia and on shed vesicles.
|
|
Research areas
|
Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
|
|
Relevance
|
Fibroblast Activation Protein alpha subunit (FAPa or FAP, alpha) is a integral membrane gelatinase belonging to the serine protease family. FAPa is the alpha subunit and DPP4 (CD26) the beta subunit of a heterodimeric membrane-bound proteinase complex also known as 170 kDa Melanoma Membrane Gelatinase, Integral Membrane Serine Proteinase and Seprase. Some cells make only FAPa homodimers, some only DPP4 homodimers. The monomer is inactive. FAP, alpha is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.
|
|
|
Fibroblast activation protein, alpha antibody - Stalk region (ab28244) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Fibroblast activation protein, alpha antibody - Stalk region is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Fibroblast activation protein, alpha antibody - Stalk region images, references, products related to ab28244 and other tools.
|
| |
Fibroblast activation protein, alpha antibody - Stalk region images:
|
 Western blot - Fibroblast activation protein, alpha antibody - Stalk region (ab28244)
All lanes : Fibroblast activation protein, alpha antibody - Stalk region (ab28244) at 1/1000 dilution
Lane 1 : Recombinant Human FAPalpha at 0.08 µg
Lane 2 : Recombinant Human FAPalpha at 0.04 µg
Lane 3 : Recombinant Human FAPalpha at 0.02 µg
Lane 4 : Recombinant Human FAPalpha at 0.01 µg
Lane 5 : Recombinant Human FAPalpha at 0.005 µg
Predicted band size : 88 kDa
|
| |
Immunocytochemistry/ Immunofluorescence-Fibroblast activation protein, alpha antibody - Stalk region(ab28244)
ICC/IF image of ab28244 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28244, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
|
| |
References for Fibroblast activation protein, alpha antibody - Stalk region (ab28244)
|
|
This product has been used in:
Bhati R et al. Molecular characterization of human breast tumor vascular cells. Am J Pathol 172:1381-90 (2008). IHC-P; Human. PubMed: 18403594
If you publish research using ab28244 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Fibroblast activation protein, alpha
|
| |
Fibroblast activation protein, alpha antibody - Stalk region - more information
|
Customer reviews (feedback) regarding Fibroblast activation protein, alpha antibody - Stalk region |
Customer FAQs regarding Fibroblast activation protein, alpha antibody - Stalk region |
Protocols for Fibroblast activation protein, alpha antibody - Stalk region |
Price and availability of products related to Fibroblast activation protein, alpha antibody - Stalk region |
| |
Fibroblast activation protein, alpha antibody - Stalk region - related products:
|
|
|
| |
Fibroblast activation protein, alpha antibody - Stalk region - other tools:
|
Contact Abcam with a Technical Enquiry about Fibroblast activation protein, alpha antibody - Stalk region |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
