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Read our guarantee »Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
Anti-Fibroblast activation protein, alpha antibody - Stalk region
See all Fibroblast activation protein, alpha products (6) ...
Rabbit polyclonal to Fibroblast activation protein, alpha - Stalk region
This antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein, alpha but does not recognize other DPP family members.
ICC/IF, IHC-P, Flow Cyt, WBmore details
Reacts with
Mouse, Rat, Human
Synthetic peptide of Human Fibroblast activation protein, alpha, corresponding to the stalk region.
(Peptide available as ab41274.)
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
Our Abpromise guarantee covers the use of ab28244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 10 µg/ml.
IHC-P: Use at an assay dependent dilution.
Flow Cyt: 1/50(PubMed: 20361950)
WB: 1/1000 - 1/5000.Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa).(1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.)
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
Fibroblast specific.
Belongs to the peptidase S9B family.
N-glycosylated.
The N-terminus may be blocked.
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
Target information above from: UniProt accessionQ12884
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Fibroblast activation protein, alpha antibody - Stalk region (ab28244)

All lanes : Anti-Fibroblast activation protein, alpha antibody - Stalk region (ab28244) at 1/1000 dilution
Lane 1 : Recombinant Human FAPalpha at 0.08 µg
Lane 2 : Recombinant Human FAPalpha at 0.04 µg
Lane 3 : Recombinant Human FAPalpha at 0.02 µg
Lane 4 : Recombinant Human FAPalpha at 0.01 µg
Lane 5 : Recombinant Human FAPalpha at 0.005 µg
Predicted band size : 88 kDa
Immunocytochemistry/ Immunofluorescence - Fibroblast activation protein, alpha antibody - Stalk region (ab28244)

ICC/IF image of ab28244 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28244, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab28244? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-Fibroblast activation protein, alpha antibody - Stalk region (ab28244) at 1/1000 dilution
Lane 1 : Recombinant Human FAPalpha at 0.08 µg
Lane 2 : Recombinant Human FAPalpha at 0.04 µg
Lane 3 : Recombinant Human FAPalpha at 0.02 µg
Lane 4 : Recombinant Human FAPalpha at 0.01 µg
Lane 5 : Recombinant Human FAPalpha at 0.005 µg
Predicted band size : 88 kDa

ICC/IF image of ab28244 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28244, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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