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Western blot - Fibroblast activation protein, alpha antibody (ab53066)
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Immunohistochemistry (Paraffin-embedded sections) - Fibroblast activation protein, alpha antibody (ab53066)
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Product Name
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Fibroblast activation protein, alpha antibody
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See all Fibroblast activation protein, alpha antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Fibroblast activation protein, alpha
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Immunogen
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A synthetic peptide derived from human Fibroblast activation protein, alpha.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab53066 detects endogenous levels of total Fibroblast activation protein, alpha.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at an assay dependent concentration.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 90 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast carcinoma tissue and LOVO cell extracts.
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Cellular localization
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Cell Membrane; single-pass type II membrane protein. Found in cell surface lamellipodia, invadopodia and on shed vesicles.
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Research areas
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Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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Relevance
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Fibroblast Activation Protein alpha subunit (FAPa or FAP, alpha) is a integral membrane gelatinase belonging to the serine protease family. FAPa is the alpha subunit and DPP4 (CD26) the beta subunit of a heterodimeric membrane-bound proteinase complex also known as 170 kDa Melanoma Membrane Gelatinase, Integral Membrane Serine Proteinase and Seprase. Some cells make only FAPa homodimers, some only DPP4 homodimers. The monomer is inactive. FAP, alpha is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.
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Fibroblast activation protein, alpha antibody (ab53066) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab53066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Fibroblast activation protein, alpha antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Fibroblast activation protein, alpha antibody images, references, products related to ab53066 and other tools.
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Fibroblast activation protein, alpha antibody images:
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 Western blot - Fibroblast activation protein, alpha antibody (ab53066)
All lanes : Fibroblast activation protein, alpha antibody (ab53066) at 1/500 dilution
Lane 1 : LOVO cell extract
Lane 2 : LOVO cell extract with immunising peptide
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Paraffin-embedded sections) - Fibroblast activation protein, alpha antibody (ab53066)
ab53066 at 1/50 dilution staining Fibroblast activation protein, alpha in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
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References for Fibroblast activation protein, alpha antibody (ab53066)
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This product has been used in: (two most recent references)
Chen H et al. TGF-beta-induced fibroblast activation protein expression, fibroblast activation protein expression increases the proliferation, adhesion, and migration of HO-8910PM. Exp Mol Pathol 87:189-94 (2009). WB, ICC/IF; Mouse. PubMed: 19747910
Santos AM et al. Targeting fibroblast activation protein inhibits tumor stromagenesis and growth in mice. J Clin Invest 119:3613-25 (2009). IHC-P; Mouse. PubMed: 19920354
See all 3 publication references for this product.
If you publish research using ab53066 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Fibroblast activation protein, alpha
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