Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
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ab54651 has been referenced in 1 publications.
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Predicted band size : 88 kDa
Fibroblast activation protein alpha antibody (ab54651) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
Overlay histogram showing HepG2 cells stained with ab54651 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54651, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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