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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
Overview
Product name
Anti-Fibroblast activation protein, alpha antibody
See all Fibroblast activation protein, alpha products (6) ...
Description
Mouse monoclonal to Fibroblast activation protein, alpha
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: PPQFDRSKKY PLLIQVYGGP CSQSVRSVFA VNWISYLASK EGMVIALVDG RGTAFQGDKL LYAVYRKLGV YEVEDQITAV RKFIEMGFID EKRIAIWGWS , corresponding to amino acids 525-625 of Human Fibroblast activation protein, alpha
PPQFDRSKKY PLLIQVYGGP CSQSVRSVFA VNWISYLASK EGMVIALVDG RGTAFQGDKL LYAVYRKLGV YEVEDQITAV RKFIEMGFID EKRIAIWGWS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
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Western blot - Fibroblast activation protein, alpha antibody (ab54651)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
Tissue specificity
Fibroblast specific.
Sequence similarities
Belongs to the peptidase S9B family.
Post-translational
modifications
N-glycosylated.
The N-terminus may be blocked.
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
Target information above from: UniProt accessionQ12884
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 170 kDa melanoma membrane bound gelatinase antibody
- DPPIV antibody
- Integral membrane serine protease antibody
see all
Database links
- Entrez Gene: 2191 Human
- Omim: 600403 Human
- SwissProt: Q12884 Human
- Unigene: 654370 Human
Anti-Fibroblast activation protein, alpha antibody images:
Western blot - Fibroblast activation protein, alpha antibody (ab54651)
Predicted band size : 88 kDa
Fibroblast activation protein alpha antibody (ab54651) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
References for Anti-Fibroblast activation protein, alpha antibody (ab54651)
This product has been referenced in:
- Lu Cet al. Dipeptidyl Peptidases as Survival Factors in Ewing Sarcoma Family of Tumors: IMPLICATIONS FOR TUMOR BIOLOGY AND THERAPY. J Biol Chem 286:27494-505 (2011). WB; Human.Read more (PubMed: 21680731) »
See 1 publication for this product
Publishing research using ab54651? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"