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Western blot - Fibroblast activation protein alpha antibody (ab54651)
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Product Name
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Fibroblast activation protein, alpha antibody
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See all Fibroblast activation protein, alpha antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Fibroblast activation protein, alpha
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Immunogen
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Recombinant fragment: PPQFDRSKKY PLLIQVYGGP CSQSVRSVFA VNWISYLASK EGMVIALVDG RGTAFQGDKL LYAVYRKLGV YEVEDQITAV RKFIEMGFID EKRIAIWGWS , corresponding to amino acids 525-625 of Human Fibroblast activation protein, alpha
BLAST 'PPQFDRSKKY PLLIQVYGGP CSQSVRSVFA VNWISYLASK EGMVIALVDG RGTAFQGDKL LYAVYRKLGV YEVEDQITAV RKFIEMGFID EKRIAIWGWS ' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; single-pass type II membrane protein. Found in cell surface lamellipodia, invadopodia and on shed vesicles.
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Research areas
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Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
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Relevance
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Fibroblast Activation Protein alpha subunit (FAPa or FAP, alpha) is a integral membrane gelatinase belonging to the serine protease family. FAPa is the alpha subunit and DPP4 (CD26) the beta subunit of a heterodimeric membrane-bound proteinase complex also known as 170 kDa Melanoma Membrane Gelatinase, Integral Membrane Serine Proteinase and Seprase. Some cells make only FAPa homodimers, some only DPP4 homodimers. The monomer is inactive. FAP, alpha is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Fibroblast activation protein, alpha antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Fibroblast activation protein, alpha antibody images:
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 Western blot - Fibroblast activation protein alpha antibody (ab54651)
Predicted band size : 88 kDa
Fibroblast activation protein alpha antibody (ab54651) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
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Search PubMed (MEDLINE) for references to Fibroblast activation protein, alpha
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