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Anti-Fibronectin Cell Binding Site antibody [3E3] (ab78030)

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1 question for ab78030

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Question 1

Wednesday 16-May-2012

I hope you are well.

I would like your advise on suitable antibodies for ELISA type assay for staining cell adhesion binding domains (e.g. 'R-G-D' peptide sequence) on fibronectin and vitronectin proteins that have been adsorbed to a surface. Depending on the surface, different adhesion domains will be exposed.

I look forward to hearing from you.

Kind regards,

ANSWER:

 

Thank you for your inquiry.

Could you please give more information about the specificity of the antibodies you are looking for? Could you provide the amino acid region the "R-G-D" peptide is contained in?

These antibodies might be the right product for your purposes, but please double check the datasheet:

ab64713

http://www.abcam.com/index.html?datasheet=64713 (or use the following: http://www.abcam.com/index.html?datasheet=64713).

ab78030

http://www.abcam.com/index.html?datasheet=78030 (or use the following: http://www.abcam.com/index.html?datasheet=78030).

Unfortunately, we do not have anti Vitronectin antibodies that are tested to inhibit binding as do the two above antibodies and I am therefore unsure which would be a suitable product for your purposes. If you provide the amino acid region and the species of theplanned sample, I will be happy to make a suggestion for you.

I am sorry I could not completely answer your questions on this occasion and hope this information is nevertheless helpful.

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