Anti-Fibronectin Cell Binding Site antibody [3E3] (ab78030)
Overview
- Product nameAnti-Fibronectin Cell Binding Site antibody [3E3]See all Fibronectin Cell Binding Site primary antibodies ...
- DescriptionMouse monoclonal [3E3] to Fibronectin Cell Binding Site
- SpecificityReacts with human fibronectin and with a peptic fragment (11.5 kD) from human fibronectin, compromising the cell attachment site. It inhibits the attachment of normal rat kidney cells to human fironectin coated plastic surface.
- Tested applicationsIHC-Fr, WB, ICC, ELISA, Inhibition Assay more details
- Species reactivityReacts with: Human
- Immunogen
Full length native protein (purified) (Human) from amnion liquid
- EpitopeCell Binding Fragment
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 0.25M Sodium chloride, 0.02M PBS -
Concentration information loading... - PurityProtein G purified
- Clonality Monoclonal
- Clone number3E3
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab78030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-Fr | |
| WB | |
| ICC | |
| ELISA | |
| Inhibition Assay |
ICC: Use at a concentration of 5 - 20 µg/ml.
IHC-Fr: Use at a concentration of 5 - 20 µg/ml.Fix with acetone
Inhib: Use at an assay dependent dilution.
WB: Use at a concentration of 5 - 20 µg/ml. Detects a band of approximately 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- RelevanceFibronectin exists in 3 forms: plasma fibronectin, a soluble dimeric form that becomes incorporated into the fibrin meshwork of clots; cell-surface fibronectin, oligomers transiently attached to the cell surface; and matrix fibronectin, highly insoluble fibrils in the extracellular matrix. The 110 kDa cell-binding fragment of fibronectin serves as a substrate for cells that express the a5ß1 Integrin receptor and other Integrin receptors that recognize the Arg-Gly-Asp (RGD) sequence.
- Cellular localizationSecreted, extracellular space, extracellular matrix.
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Database links
- Entrez Gene: 2335 Human
- Omim: 135600 Human
- SwissProt: P02751 Human
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Alternative names
- CIG antibodyfibronectin 1 antibodyFINC antibody
- FN antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyMSF antibody
see all
References for Anti-Fibronectin Cell Binding Site antibody [3E3] (ab78030)
ab78030 has not yet been referenced specifically in any publications.