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Read our guarantee »Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Fibronectin
Anti-Fibronectin Cell Binding Site antibody [3E3]
See all Fibronectin Cell Binding Site products (3) ...
Mouse monoclonal [3E3] to Fibronectin Cell Binding Site
Reacts with human fibronectin and with a peptic fragment (11.5 kD) from human fibronectin, compromising the cell attachment site. It inhibits the attachment of normal rat kidney cells to human fironectin coated plastic surface.
IHC-Fr, WB, ICC, ELISA, Inhibition Assaymore details
Reacts with
Human
Full length native protein (purified) (Human) from amnion liquid
Cell Binding Fragment
Liquid
Store at +4°C.
Preservative: 0.1% Sodium Azide
Constituents: 0.25M Sodium chloride, 0.02M PBS
Concentration information loading...
Protein G purified
Monoclonal
3E3
IgG1
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Fibronectin
Our Abpromise guarantee covers the use of ab78030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
ICC: Use at a concentration of 5 - 20 µg/ml.
IHC-Fr: Use at a concentration of 5 - 20 µg/ml.Fix with acetone
Inhib: Use at an assay dependent dilution.
WB: Use at a concentration of 5 - 20 µg/ml. Detects a band of approximately 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Fibronectin exists in 3 forms: plasma fibronectin, a soluble dimeric form that becomes incorporated into the fibrin meshwork of clots; cell-surface fibronectin, oligomers transiently attached to the cell surface; and matrix fibronectin, highly insoluble fibrils in the extracellular matrix. The 110 kDa cell-binding fragment of fibronectin serves as a substrate for cells that express the a5ß1 Integrin receptor and other Integrin receptors that recognize the Arg-Gly-Asp (RGD) sequence.
Secreted, extracellular space, extracellular matrix.
ab78030 has not yet been referenced specifically in any publications.
Publishing research using ab78030? Please let us know so that we can cite the reference in this datasheet
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