You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Fibronectin Human ELISA Kit (ab108848)

Abreviews (1)Q&A (3)

Overview

  • Product nameFibronectin Human ELISA KitSee all Fibronectin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.7 µg/ml
  • Range
    0.78 µg/ml - 50 µg/ml
  • Recovery

    101 %

  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Fibronectin is a major component of the extracellular matrix and blood plasma, and is a specific ligand for several integrin adhesion receptors. Fibronectin plays an important role not only in cell adhesion and wound healing but also in embryogenesis and hematopoiesis. Fibronectin is over-expressed in cardiovascular disease states such as atherosclerosis and myocardial infarction. Reduced levels of Fibronectin have been reported in patients with Disseminated Intravascular Coagulation (DIC) and low concentrations appear to correlate with a poor prognosis.

    ab108848 Fibronectin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit employs a quantitative competitive enzyme immunoassay technique that measures human plasma, serum and cell culture supernatant Fibronectin in less than 3 hours. A murine antibody specific for Fibronectin has been pre-coated onto a 96-well microplate with removable strips. Fibronectin in standards and samples is competed by a biotinylated Fibronectin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA more details

Properties

  • RelevanceFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7 By similarity. Interacts with COMP. Interacts with S.aureus fnbA. Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth By similarity. Interacts with FST3. Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. Sulfated. It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylation sites are present in the extracelllular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2) [MIM:601894]; also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Contains 12 fibronectin type-I domains. Contains 2 fibronectin type-II domains. Contains 16 fibronectin type-III domains.
  • Cellular localizationSecreted, extracellular space, extracellular matrix
    • Alternative names
        CIGCold insoluble globulinFibronectin 1
        FINCFNFN1FNZMigration stimulating factorMSF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108848 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Fibronectin Human ELISA Kit images

      • ELISA - Fibronectin Human ELISA Kit (ab108848)
        ELISA - Fibronectin Human ELISA Kit (ab108848)
        Fibronectin Standard Curve

      Protocols

      References for Fibronectin Human ELISA Kit (ab108848)

      ab108848 has not yet been referenced specifically in any publications.

      Publishing research using ab108848 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108848 Submit a Question for ab108848

      Displaying 1 - 4 of 4 results for Abreviews and Q&A

      10
      Votes: Highest First

      Abreview for Fibronectin Human ELISA Kit

       Excellent Excellent 5/5 (Ease of Use)
      Abreviews

      Sample: Human Serum (Human plasma)
      Specification: Human plasma
      Amount of Sample: 2.5 µg
      Type of Kit Used: ELISA
      Positive control: recombinant fibronectin
      Did you use the Abcam Kit protocol?: Yes
      abreview image
      Username

      Verified customer

      Submitted Jan 15 2013

      Dears,



      Does this kit works on Urine Samples?



      Fibronectin Human ELISA Kit (ab108848)



      Urgent pls



      --

      Thank you very much for your interest our products.

      To our knowledge, ab108848 has not been tested in urine. However by participating in our AbTrial program you can now use our products in an untested application or species without financial ri...

      Read More

      Estimad@ Sr/Sra,
      Necesitaría saber si los siguientes ELISAS humanos cross-reaccionan con el ortólogo murino:
      ab133037

      ab108822

      ab108823

      ab119524

      ab108848

      ab108847

      ab...

      Read More

      Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

      El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apropia...

      Read More

      Dear technical team,

      Our customer purchased ab108848 but, there's no instruction how to reconstitute the lyophilized standard.
      (how many volume to be applied to make 100ug/ml of standard solution)
      Check this please.
      http://www.ab...

      Read More

      Thank you for your query.

      I assume you customer 's kit has the lot number 1211713 (GR68088-1)?

      I can confirm that the standard is HumanFibronectinin a buffered protein base (250 mg, lyophilized).

      It should be reconstitutedwit...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"