Fibronectin Human ELISA kit (with color giving dyes) (ab119524)
- Product nameFibronectin Human ELISA kit (with color giving dyes)See all Fibronectin kits ...
Intra-assay Sample n Mean SD CV% Overall 5.3% Inter-assay Sample n Mean SD CV% Overall 6.7%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Urine, Serum, Plasma
- Assay typeSandwich
- Sensitivity0.1 ng/ml
- Range0.31 ng/ml - 20 ng/ml
Sample specific recovery Sample type Average % Range Serum 110.6 % - %
- Species reactivityReacts with: Human
- Product overview
ab119524 is an enzyme-linked immunosorbent assay for the quantitative detection of Human Fibronectin. The Human Fibronectin ELISA is for research use only and is not for diagnostic or therapeutic procedures.
Fibronectin is a ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix.
It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis and thrombosis.
Fibronectin is secreted as a dimer with a monomer molecular weight of 220-250 kDa. Fibronectin is composed of three types of modules organized into functional domains that are resistant to proteolysis and contain binding sites for extracellular matrix proteins, cell surface receptors and circulating blood proteins.
In general, fibronectin is synthesized by and present around fibroblasts, endothelial cells, chondrocytes, glial cells, and myocytes. Fibronectin is abundant in the connective tissue matrix and in basement membranes. Extremely high levels of the glycoprotein are found in plasma. Fibronectin has been reported to play a major role in the cardiovascular system, being functionally involved in blood clotting, atherosclerosis, hypertension and myocardial infarction.
Fibronectin's importance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation. Fetal fibronectin has been described as a predictor of term lab.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 5ml Biotin-Conjugate anti-human Fibronectin polyclonal antibody 1 x 70µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human Fibronectin Standard 2 vials Polyclonal anti-human Fibronectin-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 200µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
- Research Areas
- CIGCold insoluble globulinFibronectin 1
- FINCFNFN1FNZMigration stimulating factorMSF
Our Abpromise guarantee covers the use of ab119524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Fibronectin Human ELISA kit (with color giving dyes) images
Representative standard curve for ab119524. Human Fibronectin was diluted in serial 2-fold steps in Assay Buffer (1x).
References for Fibronectin Human ELISA kit (with color giving dyes) (ab119524)
ab119524 has not yet been referenced specifically in any publications.