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Fibronectin Human ELISA kit (with color giving dyes) (ab119524)

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Overview

  • Product nameFibronectin Human ELISA kit (with color giving dyes)See all Fibronectin kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6.7%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    0.1 ng/ml
  • Range
    0.31 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 110.6 % - %

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119524 is an enzyme-linked immunosorbent assay for the quantitative detection of Human Fibronectin. The Human Fibronectin ELISA is for research use only and is not for diagnostic or therapeutic procedures.

    Fibronectin is a ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix.

    It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis and thrombosis.

    Fibronectin is secreted as a dimer with a monomer molecular weight of 220-250 kDa. Fibronectin is composed of three types of modules organized into functional domains that are resistant to proteolysis and contain binding sites for extracellular matrix proteins, cell surface receptors and circulating blood proteins.

    In general, fibronectin is synthesized by and present around fibroblasts, endothelial cells, chondrocytes, glial cells, and myocytes. Fibronectin is abundant in the connective tissue matrix and in basement membranes. Extremely high levels of the glycoprotein are found in plasma. Fibronectin has been reported to play a major role in the cardiovascular system, being functionally involved in blood clotting, atherosclerosis, hypertension and myocardial infarction.

    Fibronectin's importance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation. Fetal fibronectin has been described as a predictor of term lab.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • RelevanceFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7 By similarity. Interacts with COMP. Interacts with S.aureus fnbA. Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth By similarity. Interacts with FST3. Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. Sulfated. It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylation sites are present in the extracelllular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2) [MIM:601894]; also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Contains 12 fibronectin type-I domains. Contains 2 fibronectin type-II domains. Contains 16 fibronectin type-III domains.
  • Cellular localizationSecreted, extracellular space, extracellular matrix
    • Alternative names
        CIGCold insoluble globulinFibronectin 1
        FINCFNFN1FNZMigration stimulating factorMSF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119524 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Fibronectin Human ELISA kit (with color giving dyes) images

      • Sandwich ELISA - Fibronectin Human ELISA kit (with color giving dyes) (ab119524)
        Sandwich ELISA - Fibronectin Human ELISA kit (with color giving dyes) (ab119524)
        Representative standard curve for ab119524. Human Fibronectin was diluted in serial 2-fold steps in Assay Buffer (1x).

      Protocols

      References for Fibronectin Human ELISA kit (with color giving dyes) (ab119524)

      ab119524 has not yet been referenced specifically in any publications.

      Publishing research using ab119524 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"