Fibronectin Rat ELISA Kit (ab108850)
- Product nameFibronectin Rat ELISA KitSee all Fibronectin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Urine, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.09 ng/ml
- Range0.098 ng/ml - 100 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Rat
- Product overview
Fibronectin (FN) is a major component of the extracellular matrix and blood plasma, and is a specific ligand for several integrin adhesion receptors. Fibronectin plays an important role not only in cell adhesion and wound healing but also in embryogenesis and hematopoiesis. Fibronectin is over-expressed in cardiovascular disease states such as atherosclerosis and myocardial infarction. Reduced levels of Fibronectin have been reported in patients with Disseminated Intravascular Coagulation (DIC) and low concentrations appear to correlate with a poor prognosis.
ab108850 Fibronectin Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the detection of Rat Fibronectin in plasma, serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Fibronectin in less than 4 hours. A polyclonal antibody specific for Fibronectin has been pre-coated onto a microplate. Fibronectin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Fibronectin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Rat Fibronectin Antibody (80x) 1 x 100µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Rat Fibronectin Microplate 1 unit Rat Fibronectin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- CIGCold insoluble globulinFibronectin 1
- FINCFNFN1FNZMigration stimulating factorMSF
Our Abpromise guarantee covers the use of ab108850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Fibronectin Rat ELISA Kit (ab108850)
ab108850 has not yet been referenced specifically in any publications.