Anti-Fibronectin antibody [2F12] (ab25583)

Overview

• Product nameAnti-Fibronectin antibody [2F12]See all Fibronectin primary antibodies ...
• Description
  Mouse monoclonal [2F12] to Fibronectin
• Tested applicationsELISA, ICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  The details of the immunogen for this antibody are not available.

• Positive controlThis antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 100mM Borate buffered saline, pH 8.2
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number2F12
• IsotypeIgG1
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Stem Cells
  • Neural Stem Cells
  • Extracellular

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Fibronectin

  • Cardiovascular
  • Angiogenesis
  • Adhesion / ECM
  • Extracellular Matrix

Applications

Target

• FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
  Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
• Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
• Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesContains 12 fibronectin type-I domains.
  Contains 2 fibronectin type-II domains.
  Contains 16 fibronectin type-III domains.
• Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
• Post-translational
  modificationsSulfated.
  It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
  Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
  Phosphorylation sites are present in the extracellular medium.
  Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02751 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2335 Human
  • Omim: 135600 Human
  • SwissProt: P02751 Human
  • Unigene: 203717 Human

• Alternative names
  CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody

  DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyfibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN 1 antibodyFN antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyTransformation sensitive protein. antibodyUgl-Y3 antibody

  see all

Anti-Fibronectin antibody [2F12] images

• 

  Western blot - Fibronectin antibody [2F12] (ab25583)
  Anti-Fibronectin antibody [2F12] (ab25583) at 1/2000 dilution + TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size : 263 kDa
  Observed band size : 273 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [2F12] (ab25583)

  ICC/IF image of ab25583 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab25583 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.