You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-Fibronectin antibody [568], prediluted (ab51836)

Submit an Abreview Submit a Question

Overview

  • Product nameAnti-Fibronectin antibody [568], predilutedSee all Fibronectin primary antibodies ...
  • Description
    Mouse monoclonal [568] to Fibronectin, prediluted
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human Fibronectin from cultivated fibroblasts.

  • Positive controlKidney, skin, liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab51836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
  • Application notesIHC-P: Use neat.

    No protease pre-treatment required. To improve specificity the following procedure may be applied: After de-waxing tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS for 15 min at 37 C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
      Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
    • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
    • Involvement in diseaseGlomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
    • Sequence similaritiesContains 12 fibronectin type-I domains.
      Contains 2 fibronectin type-II domains.
      Contains 16 fibronectin type-III domains.
    • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
    • Post-translational
      modifications      Sulfated.
      It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
      Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
      Phosphorylation sites are present in the extracellular medium.
      Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.

      • Target information above from: UniProt accession P02751 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CIG antibodyCold insoluble globulin antibodyCold-insoluble globulin antibody
          DKFZp686F10164 antibodyDKFZp686H0342 antibodyDKFZp686I1370 antibodyDKFZp686O13149 antibodyED B antibodyfibronectin 1 antibodyFINC antibodyFINC_HUMAN antibodyFN 1 antibodyFN antibodyFN1 antibodyFNZ antibodyGFND antibodyGFND2 antibodyLETS antibodyMigration stimulating factor antibodyMSF antibodyTransformation sensitive protein. antibodyUgl-Y3 antibody
        see all

      References for Anti-Fibronectin antibody [568], prediluted (ab51836)

      ab51836 has not yet been referenced specifically in any publications.

      Publishing research using ab51836 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab51836 Submit a Question for ab51836

      There are currently no Abreviews or Questions for ab51836.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"