Anti-Fibronectin antibody [5G7] (HRP) (ab25467)

Overview

  • Product nameAnti-Fibronectin antibody [5G7] (HRP)See all Fibronectin primary antibodies ...
  • Description
    Mouse monoclonal [5G7] to Fibronectin (HRP)
  • ConjugationHRP
  • Tested applicationsELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab25467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/4000.

Target

  • FunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in diseaseGlomerulopathy with fibronectin deposits 2
  • Sequence similaritiesContains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications
    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylation sites are present in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN 1 antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Transformation sensitive protein. antibody
    • Ugl-Y3 antibody
    see all

References for Anti-Fibronectin antibody [5G7] (HRP) (ab25467)

ab25467 has not yet been referenced specifically in any publications.

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If you would like to coat the microtiter plate wells with your protein target, you should do that first. Adding BSA after allowing the protein target to bind the wells serves to block the wells from binding any more...

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Good luck with your experiment! I"m happy to help if you have any further questions.

Thank you for your reply.


We would not recommend diluting the antibodies before storing. Please follow the instructions as listed on each antibody's datasheet:

For ab19183, store the whole vial at 4C.


For ab254...

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Thank you for your reply.


The ab25467 is our only HRP-conjugated anti-fibronectin antibody. We have a polyclonal that is biotinylated, but I'm not sure if that is helpful. You could use a streptavidin-HRP for detection with ab6584 thoug...

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Thank you for your reply.


You can find our recommended ELISA protocols here:


http://www.abcam.com/index.html?pageconfig=popular_protocols


You will be most interested in our direct ELISA protocol.
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Thank you for your reply.


I would recommend the following antibodies for your experiments:


ab7539 - Anti-Fibrinogen (HRP)

ab19183 - Anti - Human Serum Albumin (HRP)

ab25467 - Anti -Fibronectin (HRP) <...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"